ARAF
Serine/threonine-protein kinase A-Raf
Also known as: ARAF_HUMAN, ARAF1
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- P10398
- Gene
- ARAF
- Ensembl
- ENSG00000078061
- Chromosome
- X
- Canonical length
- 606 aa
- Protein class
- Enzymes, Human disease related genes, Plasma proteins, Predicted intracellular proteins
- Subcellular location
- Plasma membrane
OverviewNCBI Gene
Enables protein serine/threonine kinase activity. Involved in negative regulation of apoptotic process; regulation of TOR signaling; and regulation of protein metabolic process. Predicted to be active in cytosol and mitochondrion. Biomarker of high grade glioma. [provided by Alliance of Genome Resources, Jul 2025]
Canonical amino-acid sequenceUniProt
606 residues, UniProt reviewed canonical sequence.
>P10398|ARAF
1 MEPPRGPPAN GAEPSRAVGT VKVYLPNKQR TVVTVRDGMS VYDSLDKALK VRGLNQDCCV
61 VYRLIKGRKT VTAWDTAIAP LDGEELIVEV LEDVPLTMHN FVRKTFFSLA FCDFCLKFLF
121 HGFRCQTCGY KFHQHCSSKV PTVCVDMSTN RQQFYHSVQD LSGGSRQHEA PSNRPLNELL
181 TPQGPSPRTQ HCDPEHFPFP APANAPLQRI RSTSTPNVHM VSTTAPMDSN LIQLTGQSFS
241 TDAAGSRGGS DGTPRGSPSP ASVSSGRKSP HSKSPAEQRE RKSLADDKKK VKNLGYRDSG
301 YYWEVPPSEV QLLKRIGTGS FGTVFRGRWH GDVAVKVLKV SQPTAEQAQA FKNEMQVLRK
361 TRHVNILLFM GFMTRPGFAI ITQWCEGSSL YHHLHVADTR FDMVQLIDVA RQTAQGMDYL
421 HAKNIIHRDL KSNNIFLHEG LTVKIGDFGL ATVKTRWSGA QPLEQPSGSV LWMAAEVIRM
481 QDPNPYSFQS DVYAYGVVLY ELMTGSLPYS HIGCRDQIIF MVGRGYLSPD LSKISSNCPK
541 AMRRLLSDCL KFQREERPLF PQILATIELL QRSLPKIERS ASEPSLHRTQ ADELPACLLS
601 AARLVPLocalizationUniProt · AlphaFold · HPA
Whether an antibody against ARAF can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.42
- Highest tissue expression
- 128 nTPM
Expression across tissuesHPA
Tissue
- skeletal muscle: 128 nTPM
- bone marrow: 97 nTPM
- tongue: 80 nTPM
- liver: 61 nTPM
- pancreas: 56 nTPM
- heart muscle: 53 nTPM
Single-cell type
- syncytiotrophoblasts: 64 nCPM
- cholangiocytes: 51 nCPM
- extravillous trophoblasts: 49 nCPM
- hepatocytes: 48 nCPM
- enterocytes: 48 nCPM
- epididymal principal cells: 48 nCPM
Immune cell
- non-classical monocyte: 68 nTPM
- eosinophil: 62 nTPM
- intermediate monocyte: 58 nTPM
- myeloid DC: 55 nTPM
- total PBMC: 51 nTPM
- basophil: 49 nTPM
Brain region
- medulla oblongata: 35 nTPM
- choroid plexus: 34 nTPM
- thalamus: 32 nTPM
- basal ganglia: 29 nTPM
- midbrain: 29 nTPM
- white matter: 28 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.33
- gnomAD pLI
- 0.96
- gnomAD missense Z
- 2.45
- DepMap mean gene effect
- 0.2
- DepMap dependency class
- none
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- MAPK cascade
- negative regulation of apoptotic process
- positive regulation of peptidyl-serine phosphorylation
- protein modification process
- regulation of proteasomal ubiquitin-dependent protein catabolic process
- regulation of TOR signaling
Molecular functions
- ATP binding
- MAP kinase kinase kinase activity
- protein kinase activity
- protein serine kinase activity
- protein serine/threonine kinase activity
- zinc ion binding
Cellular components
Protein domainsUniProt · Pfam · InterPro
- Protein kinase domain
- Serine-threonine/tyrosine-protein kinase, catalytic domain
- Protein kinase C-like, phorbol ester/diacylglycerol-binding domain
- Raf-like Ras-binding
- Serine/threonine-protein kinase, active site
- Protein kinase-like domain superfamily
- Protein kinase, ATP binding site
- Diacylglycerol/phorbol-ester binding
- Ubiquitin-like domain superfamily
- C1-like domain superfamily
- Serine/Threonine Kinases and Pseudokinases
- Phorbol esters/diacylglycerol binding domain (C1 domain)
- Raf-like Ras-binding domain
- Protein tyrosine and serine/threonine kinase
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of ARAF in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads ARAF as an antibody target. Whether an autoantibody or antibody against ARAF could matter depends on whether native ARAF is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
ARAF is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label ARAF as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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