Seroatlas · Human Serome Atlas

NELFCD

Negative elongation factor C/D

Also known as: HSPC130, NELF-C, NELF-D, NELFD_HUMAN, TH1, TH1L

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8IXH7
Gene: NELFCD
Ensembl: ENSG00000101158
Chromosome: 20
Canonical length: 590 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Nuclear bodies,Vesicles,Cytosol

OverviewNCBI Gene

The NELF complex of proteins interacts with the DSIF protein complex to repress transcriptional elongation by RNA polymerase II. The protein encoded by this gene is an essential part of the NELF complex. Alternative translation initiation site usage results in the formation of two isoforms with different N-termini. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

590 residues, UniProt reviewed canonical sequence.

>Q8IXH7|NELFCD
     1  MAGAVPGAIM DEDYYGSAAE WGDEADGGQQ EDDSGEGEDD AEVQQECLHK FSTRDYIMEP
    61  SIFNTLKRYF QAGGSPENVI QLLSENYTAV AQTVNLLAEW LIQTGVEPVQ VQETVENHLK
   121  SLLIKHFDPR KADSIFTEEG ETPAWLEQMI AHTTWRDLFY KLAEAHPDCL MLNFTVKLIS
   181  DAGYQGEITS VSTACQQLEV FSRVLRTSLA TILDGGEENL EKNLPEFAKM VCHGEHTYLF
   241  AQAMMSVLAQ EEQGGSAVRR IAQEVQRFAQ EKGHDASQIT LALGTAASYP RACQALGAML
   301  SKGALNPADI TVLFKMFTSM DPPPVELIRV PAFLDLFMQS LFKPGARINQ DHKHKYIHIL
   361  AYAASVVETW KKNKRVSINK DELKSTSKAV ETVHNLCCNE NKGASELVAE LSTLYQCIRF
   421  PVVAMGVLKW VDWTVSEPRY FQLQTDHTPV HLALLDEIST CHQLLHPQVL QLLVKLFETE
   481  HSQLDVMEQL ELKKTLLDRM VHLLSRGYVL PVVSYIRKCL EKLDTDISLI RYFVTEVLDV
   541  IAPPYTSDFV QLFLPILEND SIAGTIKTEG EHDPVTEFIA HCKSNFIMVN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NELFCD can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.32
Highest tissue expression: 33 nTPM

Expression across tissuesHPA

Tissue

esophagus: 33 nTPM
pituitary gland: 32 nTPM
skeletal muscle: 30 nTPM
kidney: 30 nTPM
retina: 30 nTPM
thymus: 29 nTPM

Single-cell type

cytotrophoblasts: 167 nCPM
migrating cytotrophoblasts: 123 nCPM
syncytiotrophoblasts: 117 nCPM
differentiating spermatogonia: 102 nCPM
somatotrophs: 101 nCPM
undifferentiated spermatogonia: 100 nCPM

Immune cell

non-classical monocyte: 69 nTPM
myeloid DC: 65 nTPM
intermediate monocyte: 52 nTPM
memory CD8 T-cell: 51 nTPM
MAIT T-cell: 50 nTPM
total PBMC: 49 nTPM

Brain region

white matter: 29 nTPM
basal ganglia: 26 nTPM
medulla oblongata: 25 nTPM
cerebellum: 24 nTPM
thalamus: 24 nTPM
pons: 24 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.44
gnomAD pLI: 0.18
gnomAD missense Z: 2.29
DepMap mean gene effect: -0.62
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 18% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

negative regulation of transcription elongation by RNA polymerase II

Molecular functions

RNA binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

TH1 protein
TH1 protein

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NELFCD in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NELFCD as an antibody target. Whether an autoantibody or antibody against NELFCD could matter depends on whether native NELFCD is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NELFCD is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NELFCD as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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