Seroatlas · Human Serome Atlas

DBF4

Protein DBF4 homolog A

Also known as: ASK, chif, DBF4A, DBF4A_HUMAN, ZDBF1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9UBU7
Gene
DBF4
Ensembl
ENSG00000006634
Chromosome
7
Canonical length
674 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm,Nuclear bodies

OverviewNCBI Gene

Predicted to enable protein serine/threonine kinase activator activity. Predicted to be involved in positive regulation of nuclear cell cycle DNA replication and regulation of cell cycle phase transition. Located in nuclear body. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

674 residues, UniProt reviewed canonical sequence.

>Q9UBU7|DBF4
     1  MNSGAMRIHS KGHFQGGIQV KNEKNRPSLK SLKTDNRPEK SKCKPLWGKV FYLDLPSVTI
    61  SEKLQKDIKD LGGRVEEFLS KDISYLISNK KEAKFAQTLG RISPVPSPES AYTAETTSPH
   121  PSHDGSSFKS PDTVCLSRGK LLVEKAIKDH DFIPSNSILS NALSWGVKIL HIDDIRYYIE
   181  QKKKELYLLK KSSTSVRDGG KRVGSGAQKT RTGRLKKPFV KVEDMSQLYR PFYLQLTNMP
   241  FINYSIQKPC SPFDVDKPSS MQKQTQVKLR IQTDGDKYGG TSIQLQLKEK KKKGYCECCL
   301  QKYEDLETHL LSEQHRNFAQ SNQYQVVDDI VSKLVFDFVE YEKDTPKKKR IKYSVGSLSP
   361  VSASVLKKTE QKEKVELQHI SQKDCQEDDT TVKEQNFLYK ETQETEKKLL FISEPIPHPS
   421  NELRGLNEKM SNKCSMLSTA EDDIRQNFTQ LPLHKNKQEC ILDISEHTLS ENDLEELRVD
   481  HYKCNIQASV HVSDFSTDNS GSQPKQKSDT VLFPAKDLKE KDLHSIFTHD SGLITINSSQ
   541  EHLTVQAKAP FHTPPEEPNE CDFKNMDSLP SGKIHRKVKI ILGRNRKENL EPNAEFDKRT
   601  EFITQEENRI CSSPVQSLLD LFQTSEEKSE FLGFTSYTEK SGICNVLDIW EEENSDNLLT
   661  AFFSSPSTST FTGF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DBF4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.62
Highest tissue expression
14 nTPM

Expression across tissuesHPA

Tissue

  • testis: 14 nTPM
  • bone marrow: 0.6 nTPM
  • thymus: 0.5 nTPM
  • basal ganglia: 0.4 nTPM
  • lymph node: 0.4 nTPM
  • tonsil: 0.4 nTPM

Single-cell type

  • late primary spermatocytes: 1,165 nCPM
  • early spermatids: 315 nCPM
  • early primary spermatocytes: 185 nCPM
  • erythrocyte progenitors: 103 nCPM
  • late spermatids: 102 nCPM
  • monocyte progenitors: 101 nCPM

Immune cell

  • basophil: 1 nTPM
  • T-reg: 0.4 nTPM
  • gdT-cell: 0.2 nTPM
  • memory CD4 T-cell: 0.2 nTPM
  • naive B-cell: 0.2 nTPM
  • naive CD4 T-cell: 0.2 nTPM

Brain region

  • cerebellum: 2.1 nTPM
  • basal ganglia: 1.6 nTPM
  • cerebral cortex: 1.6 nTPM
  • thalamus: 1.4 nTPM
  • white matter: 1.4 nTPM
  • pons: 1.3 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.28
gnomAD pLI
0.99
gnomAD missense Z
0.98
DepMap mean gene effect
-0.87
DepMap dependency class
common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of DBF4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DBF4 as an antibody target. Whether an autoantibody or antibody against DBF4 could matter depends on whether native DBF4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DBF4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DBF4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/DBF4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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