Seroatlas · Human Serome Atlas

AKAP8

A-kinase anchor protein 8

Also known as: AKAP8_HUMAN, AKAP95, DKFZp586B1222

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O43823
Gene: AKAP8
Ensembl: ENSG00000105127
Chromosome: 19
Canonical length: 692 aa
Protein class: Plasma proteins, Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011]

Canonical amino-acid sequenceUniProt

692 residues, UniProt reviewed canonical sequence.

>O43823|AKAP8
     1  MDQGYGGYGA WSAGPANTQG AYGTGVASWQ GYENYNYYGA QNTSVTTGAT YSYGPASWEA
    61  AKANDGGLAA GAPAMHMASY GPEPCTDNSD SLIAKINQRL DMMSKEGGRG GSGGGGEGIQ
   121  DRESSFRFQP FESYDSRPCL PEHNPYRPSY SYDYEFDLGS DRNGSFGGQY SECRDPARER
   181  GSLDGFMRGR GQGRFQDRSN PGTFMRSDPF VPPAASSEPL STPWNELNYV GGRGLGGPSP
   241  SRPPPSLFSQ SMAPDYGVMG MQGAGGYDST MPYGCGRSQP RMRDRDRPKR RGFDRFGPDG
   301  TGRKRKQFQL YEEPDTKLAR VDSEGDFSEN DDAAGDFRSG DEEFKGEDEL CDSGRQRGEK
   361  EDEDEDVKKR REKQRRRDRT RDRAADRIQF ACSVCKFRSF DDEEIQKHLQ SKFHKETLRF
   421  ISTKLPDKTV EFLQEYIVNR NKKIEKRRQE LMEKETAKPK PDPFKGIGQE HFFKKIEAAH
   481  CLACDMLIPA QPQLLQRHLH SVDHNHNRRL AAEQFKKTSL HVAKSVLNNR HIVKMLEKYL
   541  KGEDPFTSET VDPEMEGDDN LGGEDKKETP EEVAADVLAE VITAAVRAVD GEGAPAPESS
   601  GEPAEDEGPT DTAEAGSDPQ AEQLLEEQVP CGTAHEKGVP KARSEAAEAG NGAETMAAEA
   661  ESAQTRVAPA PAAADAEVEQ TDAESKDAVP TE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against AKAP8 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.63
Highest tissue expression: 18 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 18 nTPM
blood vessel: 8.4 nTPM
skin: 8.3 nTPM
adipose tissue: 6.9 nTPM
esophagus: 6.8 nTPM
endometrium: 6.7 nTPM

Single-cell type

endometrial secretory cells: 290 nCPM
epididymal basal cells: 289 nCPM
endometrial glandular cells: 256 nCPM
endometrial luminal cells: 188 nCPM
syncytiotrophoblasts: 101 nCPM
epicardial cells: 91 nCPM

Immune cell

myeloid DC: 6.5 nTPM
non-classical monocyte: 6.3 nTPM
T-reg: 5.6 nTPM
memory CD8 T-cell: 4.8 nTPM
MAIT T-cell: 4.7 nTPM
intermediate monocyte: 4.6 nTPM

Brain region

cerebral cortex: 1.8 nTPM
cerebellum: 1.4 nTPM
medulla oblongata: 1.3 nTPM
hypothalamus: 1.2 nTPM
pons: 1.2 nTPM
white matter: 1.2 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.4
gnomAD pLI: 0.66
gnomAD missense Z: 0
DepMap mean gene effect: -0.07
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of AKAP8 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads AKAP8 as an antibody target. Whether an autoantibody or antibody against AKAP8 could matter depends on whether native AKAP8 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

AKAP8 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label AKAP8 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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