Seroatlas · Human Serome Atlas

SUPT16H

FACT complex subunit SPT16

Also known as: CDC68, FACT, FACTP140, FLJ10857, FLJ14010, SP16H_HUMAN, SPT16/CDC68

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9Y5B9
Gene: SUPT16H
Ensembl: ENSG00000092201
Chromosome: 14
Canonical length: 1047 aa
Protein class: Disease related genes, Enzymes, Human disease related genes, Plasma proteins, Potential drug targets, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Nucleoli fibrillar center

OverviewNCBI Gene

Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit; this gene encodes the 140 kDa subunit. [provided by RefSeq, Feb 2009]

Canonical amino-acid sequenceUniProt

1047 residues, UniProt reviewed canonical sequence.

>Q9Y5B9|SUPT16H
     1  MAVTLDKDAY YRRVKRLYSN WRKGEDEYAN VDAIVVSVGV DEEIVYAKST ALQTWLFGYE
    61  LTDTIMVFCD DKIIFMASKK KVEFLKQIAN TKGNENANGA PAITLLIREK NESNKSSFDK
   121  MIEAIKESKN GKKIGVFSKD KFPGEFMKSW NDCLNKEGFD KIDISAVVAY TIAVKEDGEL
   181  NLMKKAASIT SEVFNKFFKE RVMEIVDADE KVRHSKLAES VEKAIEEKKY LAGADPSTVE
   241  MCYPPIIQSG GNYNLKFSVV SDKNHMHFGA ITCAMGIRFK SYCSNLVRTL MVDPSQEVQE
   301  NYNFLLQLQE ELLKELRHGV KICDVYNAVM DVVKKQKPEL LNKITKNLGF GMGIEFREGS
   361  LVINSKNQYK LKKGMVFSIN LGFSDLTNKE GKKPEEKTYA LFIGDTVLVD EDGPATVLTS
   421  VKKKVKNVGI FLKNEDEEEE EEEKDEAEDL LGRGSRAALL TERTRNEMTA EEKRRAHQKE
   481  LAAQLNEEAK RRLTEQKGEQ QIQKARKSNV SYKNPSLMPK EPHIREMKIY IDKKYETVIM
   541  PVFGIATPFH IATIKNISMS VEGDYTYLRI NFYCPGSALG RNEGNIFPNP EATFVKEITY
   601  RASNIKAPGE QTVPALNLQN AFRIIKEVQK RYKTREAEEK EKEGIVKQDS LVINLNRSNP
   661  KLKDLYIRPN IAQKRMQGSL EAHVNGFRFT SVRGDKVDIL YNNIKHALFQ PCDGEMIIVL
   721  HFHLKNAIMF GKKRHTDVQF YTEVGEITTD LGKHQHMHDR DDLYAEQMER EMRHKLKTAF
   781  KNFIEKVEAL TKEELEFEVP FRDLGFNGAP YRSTCLLQPT SSALVNATEW PPFVVTLDEV
   841  ELIHFERVQF HLKNFDMVIV YKDYSKKVTM INAIPVASLD PIKEWLNSCD LKYTEGVQSL
   901  NWTKIMKTIV DDPEGFFEQG GWSFLEPEGE GSDAEEGDSE SEIEDETFNP SEDDYEEEEE
   961  DSDEDYSSEA EESDYSKESL GSEEESGKDW DELEEEARKA DRESRYEEEE EQSRSMSRKR
  1021  KASVHSSGRG SNRGSRHSSA PPKKKRK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SUPT16H can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.34
Highest tissue expression: 53 nTPM

Expression across tissuesHPA

Tissue

thymus: 53 nTPM
tonsil: 49 nTPM
lymph node: 48 nTPM
parathyroid gland: 44 nTPM
retina: 41 nTPM
testis: 41 nTPM

Single-cell type

erythrocyte progenitors: 328 nCPM
megakaryocyte progenitors: 208 nCPM
differentiating spermatogonia: 202 nCPM
monocyte progenitors: 181 nCPM
late primary spermatocytes: 173 nCPM
megakaryocyte-erythroid progenitors: 169 nCPM

Immune cell

basophil: 36 nTPM
MAIT T-cell: 35 nTPM
NK-cell: 34 nTPM
memory CD8 T-cell: 33 nTPM
naive CD8 T-cell: 32 nTPM
naive CD4 T-cell: 31 nTPM

Brain region

cerebellum: 53 nTPM
hypothalamus: 46 nTPM
cerebral cortex: 42 nTPM
midbrain: 39 nTPM
pons: 39 nTPM
white matter: 38 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about SUPT16H.

Disease | AllUniProt

Conditions SUPT16H is implicated in, by any mechanism.

Neurodevelopmental disorder with dysmorphic facies and thin corpus callosum (NEDDFAC) MIM:619480

Disease | GeneticClinVar

9 pathogenic / likely-pathogenic of 218 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Neurodevelopmental disorder with dysmorphic facies and thin corpus callosum

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.12
gnomAD pLI: 1
gnomAD missense Z: 5.1
DepMap mean gene effect: -2
DepMap dependency class: pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Peptidase M24
PH-like domain superfamily
Histone chaperone RTT106/FACT complex subunit SPT16-like, middle domain
Creatinase/Aminopeptidase P/Spt16, N-terminal
Creatinase/aminopeptidase-like
Metallopeptidase family M24
Histone chaperone Rttp106-like, middle domain
FACT complex subunit SPT16, middle domain
FACT complex subunit SPT16, N-terminal lobe domain
FACT complex subunit Spt16, peptidase M24-like domain
FACT complex subunit Spt16
FACT complex subunit SPT16, C-terminal domain
FACT complex subunit SPT16, PH-like domain
FACT complex subunit (SPT16/CDC68)
FACT complex subunit SPT16 N-terminal lobe domain
FACT complex subunit SPT16, C-terminal domain
FACT complex subunit SPT16 PH-like domain

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of SUPT16H in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SUPT16H as an antibody target. Whether an autoantibody or antibody against SUPT16H could matter depends on whether native SUPT16H is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SUPT16H is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label SUPT16H as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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