RAB5A
Ras-related protein Rab-5A
Also known as: RAB5, RAB5A_HUMAN
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- P20339
- Gene
- RAB5A
- Ensembl
- ENSG00000144566
- Chromosome
- 3
- Canonical length
- 215 aa
- Protein class
- Enzymes, Predicted intracellular proteins, RAS pathway related proteins
- Subcellular location
- Nucleoplasm,Vesicles
- Quaternary structure
- Homodimer
OverviewNCBI Gene
Enables GDP binding activity; GTP binding activity; and GTPase activity. Involved in several processes, including amyloid-beta clearance by transcytosis; early endosome to late endosome transport; and regulation of exocytosis. Located in several cellular components, including cytoplasmic side of early endosome membrane; nucleoplasm; and terminal bouton. [provided by Alliance of Genome Resources, Jul 2025]
Canonical amino-acid sequenceUniProt
215 residues, UniProt reviewed canonical sequence.
>P20339|RAB5A
1 MASRGATRPN GPNTGNKICQ FKLVLLGESA VGKSSLVLRF VKGQFHEFQE STIGAAFLTQ
61 TVCLDDTTVK FEIWDTAGQE RYHSLAPMYY RGAQAAIVVY DITNEESFAR AKNWVKELQR
121 QASPNIVIAL SGNKADLANK RAVDFQEAQS YADDNSLLFM ETSAKTSMNV NEIFMAIAKK
181 LPKNEPQNPG ANSARGRGVD LTEPTQPTRN QCCSNLocalizationUniProt · AlphaFold · HPA
Whether an antibody against RAB5A can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Cell surface
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.36
- Highest tissue expression
- 58 nTPM
Expression across tissuesHPA
Tissue
- spinal cord: 58 nTPM
- parathyroid gland: 51 nTPM
- midbrain: 50 nTPM
- amygdala: 47 nTPM
- placenta: 46 nTPM
- cerebral cortex: 46 nTPM
Single-cell type
- neutrophils: 548 nCPM
- esophageal apical cells: 511 nCPM
- salivary acinar cells: 272 nCPM
- syncytiotrophoblasts: 242 nCPM
- enterocytes: 180 nCPM
- salivary myoepithelial cells: 179 nCPM
Immune cell
- neutrophil: 36 nTPM
- eosinophil: 31 nTPM
- basophil: 29 nTPM
- non-classical monocyte: 26 nTPM
- NK-cell: 20 nTPM
- intermediate monocyte: 20 nTPM
Brain region
- white matter: 58 nTPM
- spinal cord: 54 nTPM
- medulla oblongata: 52 nTPM
- hypothalamus: 52 nTPM
- midbrain: 52 nTPM
- pons: 50 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.54
- gnomAD pLI
- 0.62
- gnomAD missense Z
- 0.85
- DepMap mean gene effect
- -0.09
- DepMap dependency class
- selective
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- amyloid-beta clearance by transcytosis
- canonical Wnt signaling pathway
- early endosome to late endosome transport
- endocytosis
- endosomal transport
- host-mediated perturbation of viral process
- intracellular protein transport
- phagocytosis
- plasma membrane to endosome transport
- positive regulation of exocytosis
- protein localization to early endosome
- receptor internalization
- regulation of autophagosome assembly
- regulation of endosome size
- regulation of filopodium assembly
- regulation of long-term neuronal synaptic plasticity
- regulation of synaptic vesicle exocytosis
- synaptic vesicle recycling
Molecular functions
Cellular components
- actin cytoskeleton
- axon
- axon terminus
- clathrin-coated endocytic vesicle membrane
- cytoplasm
- cytoplasmic side of early endosome membrane
- cytosol
- dendrite
- early endosome
- early endosome membrane
- early phagosome
- endocytic vesicle
- endomembrane system
- endosome
- endosome membrane
- extracellular exosome
- melanosome
- membrane raft
- neuronal cell body
- nucleoplasm
- phagocytic vesicle
- phagocytic vesicle membrane
- plasma membrane
- ruffle
- somatodendritic compartment
- synaptic vesicle
- synaptic vesicle membrane
- terminal bouton
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of RAB5A in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads RAB5A as an antibody target. Whether an autoantibody or antibody against RAB5A could matter depends on whether native RAB5A is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
RAB5A is annotated at the cell surface, where native RAB5A is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.
Annotation status
The present source text does not explicitly label RAB5A as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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