MAF1
Repressor of RNA polymerase III transcription MAF1 homolog
Also known as: DKFZp586G1123, MAF1_HUMAN
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- Q9H063
- Gene
- MAF1
- Ensembl
- ENSG00000179632
- Chromosome
- 8
- Canonical length
- 256 aa
- Protein class
- Predicted intracellular proteins
- Subcellular location
- Nucleoplasm,Cytosol
OverviewNCBI Gene
This gene encodes a protein that is similar to Maf1, a Saccharomyces cerevisiae protein highly conserved in eukaryotic cells. Yeast Maf1 is a negative effector of RNA polymerase III (Pol III). It responds to changes in the cellular environment and represses pol III transcription. Biochemical studies identified the initiation factor TFIIIB as a target for Maf1-dependent repression. [provided by RefSeq, Jul 2008]
Canonical amino-acid sequenceUniProt
256 residues, UniProt reviewed canonical sequence.
>Q9H063|MAF1
1 MKLLENSSFE AINSQLTVET GDAHIIGRIE SYSCKMAGDD KHMFKQFCQE GQPHVLEALS
61 PPQTSGLSPS RLSKSQGGEE EGPLSDKCSR KTLFYLIATL NESFRPDYDF STARSHEFSR
121 EPSLSWVVNA VNCSLFSAVR EDFKDLKPQL WNAVDEEICL AECDIYSYNP DLDSDPFGED
181 GSLWSFNYFF YNKRLKRIVF FSCRSISGST YTPSEAGNEL DMELGEEEVE EESRSGGSGA
241 EETSTMEEDR VPVICILocalizationUniProt · AlphaFold · HPA
Whether an antibody against MAF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.38
- Highest tissue expression
- 387 nTPM
Expression across tissuesHPA
Tissue
- skeletal muscle: 387 nTPM
- tongue: 275 nTPM
- heart muscle: 180 nTPM
- colon: 174 nTPM
- blood vessel: 156 nTPM
- bone marrow: 155 nTPM
Single-cell type
- esophageal apical cells: 210 nCPM
- late primary spermatocytes: 187 nCPM
- platelets: 162 nCPM
- esophageal suprabasal cells: 162 nCPM
- syncytiotrophoblasts: 157 nCPM
- esophageal basal cells: 154 nCPM
Immune cell
- naive B-cell: 130 nTPM
- eosinophil: 127 nTPM
- memory CD8 T-cell: 118 nTPM
- total PBMC: 112 nTPM
- naive CD4 T-cell: 111 nTPM
- memory B-cell: 111 nTPM
Brain region
- medulla oblongata: 79 nTPM
- thalamus: 74 nTPM
- basal ganglia: 73 nTPM
- white matter: 73 nTPM
- midbrain: 70 nTPM
- cerebellum: 69 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.35
- gnomAD pLI
- 0.95
- gnomAD missense Z
- 2.22
- DepMap mean gene effect
- -0.22
- DepMap dependency class
- selective
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 19% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- negative regulation of transcription by RNA polymerase I
- negative regulation of transcription by RNA polymerase III
Molecular functions
- GABA receptor binding
- RNA polymerase III type 1 promoter sequence-specific DNA binding
- RNA polymerase III type 2 promoter sequence-specific DNA binding
- RNA polymerase III type 3 promoter sequence-specific DNA binding
- RNA polymerase III core binding
Cellular components
Protein domainsUniProt · Pfam · InterPro
- Repressor of RNA polymerase III transcription Maf1
- Repressor of RNA polymerase III transcription Maf1 superfamily
- Maf1 regulator
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of MAF1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads MAF1 as an antibody target. Whether an autoantibody or antibody against MAF1 could matter depends on whether native MAF1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
MAF1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label MAF1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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