Seroatlas · Human Serome Atlas

GSPT1

Eukaryotic peptide chain release factor GTP-binding subunit ERF3A

Also known as: eRF3a, ERF3A_HUMAN, ETF3A, GST1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P15170
Gene: GSPT1
Ensembl: ENSG00000103342
Chromosome: 16
Canonical length: 499 aa
Protein class: Plasma proteins, Predicted intracellular proteins
Subcellular location: Vesicles,Cytosol

OverviewNCBI Gene

Enables GTPase activity and translation release factor activity. Involved in regulation of translational termination and translational termination. Acts upstream of or within protein methylation. Part of translation release factor complex. Is active in cytosolic ribosome. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

499 residues, UniProt reviewed canonical sequence.

>P15170|GSPT1
     1  MELSEPIVEN GETEMSPEES WEHKEEISEA EPGGGSLGDG RPPEESAHEM MEEEEEIPKP
    61  KSVVAPPGAP KKEHVNVVFI GHVDAGKSTI GGQIMYLTGM VDKRTLEKYE REAKEKNRET
   121  WYLSWALDTN QEERDKGKTV EVGRAYFETE KKHFTILDAP GHKSFVPNMI GGASQADLAV
   181  LVISARKGEF ETGFEKGGQT REHAMLAKTA GVKHLIVLIN KMDDPTVNWS NERYEECKEK
   241  LVPFLKKVGF NPKKDIHFMP CSGLTGANLK EQSDFCPWYI GLPFIPYLDN LPNFNRSVDG
   301  PIRLPIVDKY KDMGTVVLGK LESGSICKGQ QLVMMPNKHN VEVLGILSDD VETDTVAPGE
   361  NLKIRLKGIE EEEILPGFIL CDPNNLCHSG RTFDAQIVII EHKSIICPGY NAVLHIHTCI
   421  EEVEITALIC LVDKKSGEKS KTRPRFVKQD QVCIARLRTA GTICLETFKD FPQMGRFTLR
   481  DEGKTIAIGK VLKLVPEKD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against GSPT1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.3
Highest tissue expression: 120 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 120 nTPM
tongue: 91 nTPM
urinary bladder: 80 nTPM
tonsil: 77 nTPM
bone marrow: 72 nTPM
liver: 71 nTPM

Single-cell type

suprabasal keratinocytes: 343 nCPM
alveolar cells type 2: 339 nCPM
basal keratinocytes: 338 nCPM
erythrocytes: 318 nCPM
endometrial luminal cells: 308 nCPM
ocular epithelial cells: 301 nCPM

Immune cell

intermediate monocyte: 11 nTPM
T-reg: 11 nTPM
non-classical monocyte: 10 nTPM
MAIT T-cell: 8.8 nTPM
memory CD4 T-cell: 8.2 nTPM
naive CD4 T-cell: 8.2 nTPM

Brain region

cerebral cortex: 69 nTPM
white matter: 67 nTPM
choroid plexus: 66 nTPM
medulla oblongata: 63 nTPM
spinal cord: 61 nTPM
hypothalamus: 60 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.16
gnomAD pLI: 1
gnomAD missense Z: 3.32
DepMap mean gene effect: -1.71
DepMap dependency class: pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of GSPT1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads GSPT1 as an antibody target. Whether an autoantibody or antibody against GSPT1 could matter depends on whether native GSPT1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

GSPT1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label GSPT1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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