Seroatlas · Human Serome Atlas

SHFL

Shiftless antiviral inhibitor of ribosomal frameshifting protein

Also known as: C19orf66, FLJ11286, IRAV, RyDEN, SFL, SHFL_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9NUL5
Gene: SHFL
Ensembl: ENSG00000130813
Chromosome: 19
Canonical length: 291 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Cytosol

OverviewNCBI Gene

This gene is an interferon stimulated gene (ISG) that inhibits viral replication. The encoded protein binds nucleic acids and inhibits programmed -1 ribosomal frameshifting required for translation by many RNA viruses. Viruses inhibited by the protein include Zika virus, dengue virus and the coronaviruses, SARS-CoV and SARS-CoV2. [provided by RefSeq, Aug 2021]

Canonical amino-acid sequenceUniProt

291 residues, UniProt reviewed canonical sequence.

>Q9NUL5|SHFL
     1  MSQEGVELEK SVRRLREKFH GKVSSKKAGA LMRKFGSDHT GVGRSIVYGV KQKDGQELSN
    61  DLDAQDPPED MKQDRDIQAV ATSLLPLTEA NLRMFQRAQD DLIPAVDRQF ACSSCDHVWW
   121  RRVPQRKEVS RCRKCRKRYE PVPADKMWGL AEFHCPKCRH NFRGWAQMGS PSPCYGCGFP
   181  VYPTRILPPR WDRDPDRRST HTHSCSAADC YNRREPHVPG TSCAHPKSRK QNHLPKVLHP
   241  SNPHISSGST VATCLSQGGL LEDLDNLILE DLKEEEEEEE EVEDEEGGPR E

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SHFL can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.29
Highest tissue expression: 101 nTPM

Expression across tissuesHPA

Tissue

liver: 101 nTPM
choroid plexus: 26 nTPM
ovary: 24 nTPM
hypothalamus: 22 nTPM
cerebral cortex: 22 nTPM
kidney: 21 nTPM

Single-cell type

hepatocytes: 102 nCPM
late spermatids: 58 nCPM
ovarian stromal cells: 53 nCPM
breast lactating cells: 50 nCPM
decidual stromal cells: 49 nCPM
t-cells: 49 nCPM

Immune cell

NK-cell: 33 nTPM
MAIT T-cell: 31 nTPM
memory CD8 T-cell: 25 nTPM
gdT-cell: 23 nTPM
T-reg: 22 nTPM
non-classical monocyte: 20 nTPM

Brain region

hypothalamus: 15 nTPM
spinal cord: 14 nTPM
white matter: 12 nTPM
cerebral cortex: 12 nTPM
thalamus: 12 nTPM
basal ganglia: 11 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.64
gnomAD pLI: 0.14
DepMap mean gene effect: -0.12
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Shiftless antiviral inhibitor of ribosomal frameshifting
Uncharacterised protein UPF0515

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of SHFL in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SHFL as an antibody target. Whether an autoantibody or antibody against SHFL could matter depends on whether native SHFL is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SHFL is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label SHFL as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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