Seroatlas · Human Serome Atlas

DDX20

Probable ATP-dependent RNA helicase DDX20

Also known as: DDX20_HUMAN, DP103, GEMIN3

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9UHI6
Gene: DDX20
Ensembl: ENSG00000064703
Chromosome: 1
Canonical length: 824 aa
Protein class: Enzymes, Plasma proteins, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Nuclear bodies,Cytosol

OverviewNCBI Gene

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which has an ATPase activity and is a component of the survival of motor neurons (SMN) complex. This protein interacts directly with SMN, the spinal muscular atrophy gene product, and may play a catalytic role in the function of the SMN complex on RNPs. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

824 residues, UniProt reviewed canonical sequence.

>Q9UHI6|DDX20
     1  MAAAFEASGA LAAVATAMPA EHVAVQVPAP EPTPGPVRIL RTAQDLSSPR TRTGDVLLAE
    61  PADFESLLLS RPVLEGLRAA GFERPSPVQL KAIPLGRCGL DLIVQAKSGT GKTCVFSTIA
   121  LDSLVLENLS TQILILAPTR EIAVQIHSVI TAIGIKMEGL ECHVFIGGTP LSQDKTRLKK
   181  CHIAVGSPGR IKQLIELDYL NPGSIRLFIL DEADKLLEEG SFQEQINWIY SSLPASKQML
   241  AVSATYPEFL ANALTKYMRD PTFVRLNSSD PSLIGLKQYY KVVNSYPLAH KVFEEKTQHL
   301  QELFSRIPFN QALVFSNLHS RAQHLADILS SKGFPAECIS GNMNQNQRLD AMAKLKHFHC
   361  RVLISTDLTS RGIDAEKVNL VVNLDVPLDW ETYMHRIGRA GRFGTLGLTV TYCCRGEEEN
   421  MMMRIAQKCN INLLPLPDPI PSGLMEECVD WDVEVKAAVH TYGIASVPNQ PLKKQIQKIE
   481  RTLQIQKAHG DHMASSRNNS VSGLSVKSKN NTKQKLPVKS HSECGIIEKA TSPKELGCDR
   541  QSEEQMKNSV QTPVENSTNS QHQVKEALPV SLPQIPCLSS FKIHQPYTLT FAELVEDYEH
   601  YIKEGLEKPV EIIRHYTGPG DQTVNPQNGF VRNKVIEQRV PVLASSSQSG DSESDSDSYS
   661  SRTSSQSKGN KSYLEGSSDN QLKDSESTPV DDRISLEQPP NGSDTPNPEK YQESPGIQMK
   721  TRLKEGASQR AKQSRRNLPR RSSFRLQTEA QEDDWYDCHR EIRLSFSDTY QDYEEYWRAY
   781  YRAWQEYYAA ASHSYYWNAQ RHPSWMAAYH MNTIYLQEMM HSNQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DDX20 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.44
Highest tissue expression: 38 nTPM

Expression across tissuesHPA

Tissue

testis: 38 nTPM
skeletal muscle: 5 nTPM
tonsil: 3.9 nTPM
thymus: 3.4 nTPM
breast: 3.1 nTPM
fallopian tube: 2.9 nTPM

Single-cell type

late spermatids: 1,200 nCPM
early spermatids: 815 nCPM
late primary spermatocytes: 481 nCPM
cardiomyocytes: 137 nCPM
early primary spermatocytes: 30 nCPM
erythrocyte progenitors: 29 nCPM

Immune cell

basophil: 15 nTPM
MAIT T-cell: 11 nTPM
naive CD4 T-cell: 11 nTPM
T-reg: 10 nTPM
memory B-cell: 9.4 nTPM
memory CD4 T-cell: 9.2 nTPM

Brain region

white matter: 4.6 nTPM
cerebral cortex: 3.8 nTPM
hippocampal formation: 3.6 nTPM
midbrain: 3.6 nTPM
amygdala: 3.5 nTPM
hypothalamus: 3.4 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.04
gnomAD pLI: 0
gnomAD missense Z: 0.08
DepMap mean gene effect: -1.3
DepMap dependency class: pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of DDX20 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DDX20 as an antibody target. Whether an autoantibody or antibody against DDX20 could matter depends on whether native DDX20 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DDX20 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DDX20 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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