Seroatlas · Human Serome Atlas

MFSD4A

Major facilitator superfamily domain-containing protein 4A

Also known as: DKFZp761N1114, FLJ25004, FLJ34577, MFD4A_HUMAN, MFSD4, SLC60A1, UNQ3064

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8N468
Gene: MFSD4A
Ensembl: ENSG00000174514
Chromosome: 1
Canonical length: 514 aa
Protein class: Predicted intracellular proteins, Predicted membrane proteins, Transporters

OverviewNCBI Gene

Predicted to enable transmembrane transporter activity. Predicted to be involved in transmembrane transport. Predicted to be located in membrane. Biomarker of cholangiocarcinoma. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

514 residues, UniProt reviewed canonical sequence.

>Q8N468|MFSD4A
     1  MGCDGRVSGL LRRNLQPTLT YWSVFFSFGL CIAFLGPTLL DLRCQTHSSL PQISWVFFSQ
    61  QLCLLLGSAL GGVFKRTLAQ SLWALFTSSL AISLVFAVIP FCRDVKVLAS VMALAGLAMG
   121  CIDTVANMQL VRMYQKDSAV FLQVLHFFVG FGALLSPLIA DPFLSEANCL PANSTANTTS
   181  RGHLFHVSRV LGQHHVDAKP WSNQTFPGLT PKDGAGTRVS YAFWIMALIN LPVPMAVLML
   241  LSKERLLTCC PQRRPLLLSA DELALETQPP EKEDASSLPP KFQSHLGHED LFSCCQRKNL
   301  RGAPYSFFAI HITGALVLFM TDGLTGAYSA FVYSYAVEKP LSVGHKVAGY LPSLFWGFIT
   361  LGRLLSIPIS SRMKPATMVF INVVGVVVTF LVLLIFSYNV VFLFVGTASL GLFLSSTFPS
   421  MLAYTEDSLQ YKGCATTVLV TGAGVGEMVL QMLVGSIFQA QGSYSFLVCG VIFGCLAFTF
   481  YILLLFFHRM HPGLPSVPTQ DRSIGMENSE CYQR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MFSD4A can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Unknown
Secreted: No
Transmembrane segments: 12
Mean surface accessibility (rSASA): 0.33
Highest tissue expression: 125 nTPM

Expression across tissuesHPA

Tissue

stomach: 125 nTPM
kidney: 62 nTPM
cerebral cortex: 32 nTPM
cerebellum: 24 nTPM
rectum: 17 nTPM
salivary gland: 16 nTPM

Single-cell type

parietal cells: 918 nCPM
retinal bipolar cells: 239 nCPM
loop of henle epithelial cells: 130 nCPM
distal convoluted tubule cells: 125 nCPM
endometrial glandular cells: 113 nCPM
salivary duct cells: 110 nCPM

Immune cell

naive B-cell: 0.1 nTPM
basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM

Brain region

cerebral cortex: 76 nTPM
hippocampal formation: 68 nTPM
amygdala: 62 nTPM
white matter: 49 nTPM
basal ganglia: 48 nTPM
cerebellum: 29 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.7
gnomAD pLI: 0
DepMap mean gene effect: 0.02
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Molecular functions

transmembrane transporter activity

Cellular components

membrane

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MFSD4A in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MFSD4A as an antibody target. Whether an autoantibody or antibody against MFSD4A could matter depends on whether native MFSD4A is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MFSD4A is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MFSD4A as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...