Seroatlas · Human Serome Atlas

ARF6

ADP-ribosylation factor 6

Also known as: ARF6_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P62330
Gene: ARF6
Ensembl: ENSG00000165527
Chromosome: 14
Canonical length: 175 aa
Protein class: Enzymes, Predicted intracellular proteins, RAS pathway related proteins
Subcellular location: Cytosol
Quaternary structure: Homodimer

OverviewNCBI Gene

This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

175 residues, UniProt reviewed canonical sequence.

>P62330|ARF6
     1  MGKVLSKIFG NKEMRILMLG LDAAGKTTIL YKLKLGQSVT TIPTVGFNVE TVTYKNVKFN
    61  VWDVGGQDKI RPLWRHYYTG TQGLIFVVDC ADRDRIDEAR QELHRIINDR EMRDAIILIF
   121  ANKQDLPDAM KPHEIQEKLG LTRIRDRNWY VQPSCATSGD GLYEGLTWLT SNYKS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ARF6 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Cell surface
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.27
Highest tissue expression: 82 nTPM

Expression across tissuesHPA

Tissue

esophagus: 82 nTPM
bone marrow: 78 nTPM
tonsil: 64 nTPM
lymph node: 58 nTPM
rectum: 51 nTPM
skin: 51 nTPM

Single-cell type

esophageal apical cells: 2,406 nCPM
esophageal suprabasal cells: 540 nCPM
suprabasal keratinocytes: 502 nCPM
basal keratinocytes: 459 nCPM
epididymal basal cells: 298 nCPM
ocular epithelial cells: 286 nCPM

Immune cell

MAIT T-cell: 42 nTPM
memory CD8 T-cell: 38 nTPM
basophil: 37 nTPM
T-reg: 37 nTPM
memory B-cell: 37 nTPM
memory CD4 T-cell: 36 nTPM

Brain region

choroid plexus: 27 nTPM
thalamus: 27 nTPM
spinal cord: 26 nTPM
pons: 25 nTPM
white matter: 25 nTPM
cerebellum: 25 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.72
gnomAD pLI: 0.72
gnomAD missense Z: 2.78
DepMap mean gene effect: -0.29
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ARF6 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ARF6 as an antibody target. Whether an autoantibody or antibody against ARF6 could matter depends on whether native ARF6 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ARF6 is annotated at the cell surface, where native ARF6 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label ARF6 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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