ASAP2

Protein identityUniProt · HPA

UniProt accession

O43150

Gene

ASAP2

Ensembl

ENSG00000151693

Chromosome

2

Canonical length

1006 aa

Protein class

Predicted intracellular proteins

Subcellular location

Plasma membrane,Cytosol

OverviewNCBI Gene

This gene encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal Src homology 3 (SH3) domain. The protein localizes in the Golgi apparatus and at the plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta (PYK2). The encoded protein forms a stable complex with PYK2 in vivo. This interaction appears to be mediated by binding of its SH3 domain to the C-terminal proline-rich domain of PYK2. The encoded protein is tyrosine phosphorylated by activated PYK2. It has catalytic activity for class I and II ArfGAPs in vitro, and can bind the class III Arf ARF6 without immediate GAP activity. The encoded protein is believed to function as an ARF GAP that controls ARF-mediated vesicle budding when recruited to Golgi membranes. In addition, it functions as a substrate and downstream target for PYK2 and SRC, a pathway that may be involved in the regulation of vesicular transport. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Canonical amino-acid sequenceUniProt

1006 residues, UniProt reviewed canonical sequence.

>O43150|ASAP2
     1  MPDQISVSEF VAETHEDYKA PTASSFTTRT AQCRNTVAAI EEALDVDRMV LYKMKKSVKA
    61  INSSGLAHVE NEEQYTQALE KFGGNCVCRD DPDLGSAFLK FSVFTKELTA LFKNLIQNMN
   121  NIISFPLDSL LKGDLKGVKG DLKKPFDKAW KDYETKITKI EKEKKEHAKL HGMIRTEISG
   181  AEIAEEMEKE RRFFQLQMCE YLLKVNEIKI KKGVDLLQNL IKYFHAQCNF FQDGLKAVES
   241  LKPSIETLST DLHTIKQAQD EERRQLIQLR DILKSALQVE QKEDSQIRQS TAYSLHQPQG
   301  NKEHGTERNG SLYKKSDGIR KVWQKRKCSV KNGFLTISHG TANRPPAKLN LLTCQVKTNP
   361  EEKKCFDLIS HDRTYHFQAE DEQECQIWMS VLQNSKEEAL NNAFKGDDNT GENNIVQELT
   421  KEIISEVQRM TGNDVCCDCG APDPTWLSTN LGILTCIECS GIHRELGVHY SRMQSLTLDV
   481  LGTSELLLAK NIGNAGFNEI MECCLPAEDS VKPNPGSDMN ARKDYITAKY IERRYARKKH
   541  ADNAAKLHSL CEAVKTRDIF GLLQAYADGV DLTEKIPLAN GHEPDETALH LAVRSVDRTS
   601  LHIVDFLVQN SGNLDKQTGK GSTALHYCCL TDNAECLKLL LRGKASIEIA NESGETPLDI
   661  AKRLKHEHCE ELLTQALSGR FNSHVHVEYE WRLLHEDLDE SDDDMDEKLQ PSPNRREDRP
   721  ISFYQLGSNQ LQSNAVSLAR DAANLAKEKQ RAFMPSILQN ETYGALLSGS PPPAQPAAPS
   781  TTSAPPLPPR NVGKVQTASS ANTLWKTNSV SVDGGSRQRS SSDPPAVHPP LPPLRVTSTN
   841  PLTPTPPPPV AKTPSVMEAL SQPSKPAPPG ISQIRPPPLP PQPPSRLPQK KPAPGADKST
   901  PLTNKGQPRG PVDLSATEAL GPLSNAMVLQ PPAPMPRKSQ ATKLKPKRVK ALYNCVADNP
   961  DELTFSEGDV IIVDGEEDQE WWIGHIDGDP GRKGAFPVSF VHFIAD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ASAP2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.42

Highest tissue expression

44 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.22

gnomAD pLI

1

gnomAD missense Z

2.37

DepMap mean gene effect

0.13

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Molecular functions

• GTPase activator activity
• metal ion binding

Cellular components

• cytosol
• Golgi cisterna membrane
• plasma membrane

Protein domainsUniProt · Pfam · InterPro

• Arf GTPase activating protein
• SH3 domain
• Pleckstrin homology domain
• Ankyrin repeat
• BAR domain
• PH-like domain superfamily
• AH/BAR domain superfamily
• SH3-like domain superfamily
• Ankyrin repeat-containing domain superfamily
• ARFGAP/RecO-like zinc finger
• ASAP, PH domain
• ArfGAP domain superfamily
• Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein
• PH domain
• Putative GTPase activating protein for Arf
• Ankyrin repeats (3 copies)
• Variant SH3 domain
• BAR domain of APPL family
• ASAP2, SH3 domain

KeywordsUniProt

• ANK repeat
• Cell membrane
• Coiled coil
• Cytoplasm
• Golgi apparatus
• GTPase activation
• Membrane
• Metal-binding
• Phosphoprotein
• Repeat
• SH3 domain
• Zinc

InteractionsUniProt · HPA

Protein binding partners of ASAP2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• PTK2B
• ARF6
• ARF1
• PXN
• GRB2
• SH3KBP1