RALBP1

Protein identityUniProt · HPA

UniProt accession

Q15311

Gene

RALBP1

Ensembl

ENSG00000017797

Chromosome

18

Canonical length

655 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins, RAS pathway related proteins, Transporters

Subcellular location

Nucleoplasm,Nuclear bodies,Plasma membrane

OverviewNCBI Gene

RALBP1 plays a role in receptor-mediated endocytosis and is a downstream effector of the small GTP-binding protein RAL (see RALA; MIM 179550). Small G proteins, such as RAL, have GDP-bound inactive and GTP-bound active forms, which shift from the inactive to the active state through the action of RALGDS (MIM 601619), which in turn is activated by RAS (see HRAS; MIM 190020) (summary by Feig, 2003 [PubMed 12888294]).[supplied by OMIM, Nov 2010]

Canonical amino-acid sequenceUniProt

655 residues, UniProt reviewed canonical sequence.

>Q15311|RALBP1
     1  MTECFLPPTS SPSEHRRVEH GSGLTRTPSS EEISPTKFPG LYRTGEPSPP HDILHEPPDV
    61  VSDDEKDHGK KKGKFKKKEK RTEGYAAFQE DSSGDEAESP SKMKRSKGIH VFKKPSFSKK
   121  KEKDFKIKEK PKEEKHKEEK HKEEKHKEKK SKDLTAADVV KQWKEKKKKK KPIQEPEVPQ
   181  IDVPNLKPIF GIPLADAVER TMMYDGIRLP AVFRECIDYV EKYGMKCEGI YRVSGIKSKV
   241  DELKAAYDRE ESTNLEDYEP NTVASLLKQY LRDLPENLLT KELMPRFEEA CGRTTETEKV
   301  QEFQRLLKEL PECNYLLISW LIVHMDHVIA KELETKMNIQ NISIVLSPTV QISNRVLYVF
   361  FTHVQELFGN VVLKQVMKPL RWSNMATMPT LPETQAGIKE EIRRQEFLLN CLHRDLQGGI
   421  KDLSKEERLW EVQRILTALK RKLREAKRQE CETKIAQEIA SLSKEDVSKE EMNENEEVIN
   481  ILLAQENEIL TEQEELLAME QFLRRQIASE KEEIERLRAE IAEIQSRQQH GRSETEEYSS
   541  ESESESEDEE ELQIILEDLQ RQNEELEIKN NHLNQAIHEE REAIIELRVQ LRLLQMQRAK
   601  AEQQAQEDEE PEWRGGAVQP PRDGVLEPKA AKEQPKAGKE PAKPSPSRDR KETSI

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RALBP1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.49

Highest tissue expression

70 nTPM

Expression across tissuesHPA

ReferencesPubMed · IEDB

Publications for RALBP1 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.45

gnomAD pLI

0.15

gnomAD missense Z

2.01

DepMap mean gene effect

-0.09

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• chemotaxis
• positive regulation of GTPase activity
• positive regulation of mitochondrial fission
• positive regulation of protein phosphorylation
• receptor-mediated endocytosis
• regulation of Cdc42 protein signal transduction
• regulation of GTPase activity
• regulation of small GTPase mediated signal transduction
• small GTPase-mediated signal transduction
• transmembrane transport
• xenobiotic detoxification by transmembrane export across the plasma membrane
• doxorubicin transport

Molecular functions

• ABC-type glutathione S-conjugate transporter activity
• ABC-type xenobiotic transporter activity
• ATP binding
• ATPase-coupled transmembrane transporter activity
• GTPase activator activity
• small GTPase binding
• transmembrane transporter activity
• xenobiotic transmembrane transporter activity

Cellular components

• cytosol
• membrane
• mitochondrion
• nuclear body
• nucleoplasm
• plasma membrane
• spindle pole

Protein domainsUniProt · Pfam · InterPro

• Rho GTPase-activating protein domain
• Rho GTPase activation protein
• RhoGAP domain
• RalA-binding protein 1
• RalA-binding protein 1-like, Ral binding domain
• RLIP76, Ral binding domain

KeywordsUniProt

• Acetylation
• ATP-binding
• Cell membrane
• Cytoplasm
• Cytoskeleton
• GTPase activation
• Membrane
• Mitochondrion
• Nucleotide-binding
• Nucleus
• Phosphoprotein
• Translocase
• Transport

InteractionsUniProt · HPA

Protein binding partners of RALBP1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• AP2M1
• CDC42
• DNM1L
• EPN1
• NUMB
• RAC1
• REPS1
• REPS2
• TFAP2A
• AAK1
• AP2S1
• BMP2K
• CBY1
• CCDC6
• CREBZF
• DYRK1A
• GPS2
• GSE1
• HOOK2
• MSANTD4
• PPL
• RALA
• RALB
• SAPCD2
• TFPT