Seroatlas · Human Serome Atlas

PRPF4

U4/U6 small nuclear ribonucleoprotein Prp4

Also known as: HPRP4, HPRP4P, PRP4, PRP4_HUMAN, Prp4p, SNRNP60

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O43172
Gene
PRPF4
Ensembl
ENSG00000136875
Chromosome
9
Canonical length
522 aa
Protein class
Disease related genes, Human disease related genes, Plasma proteins, Predicted intracellular proteins
Subcellular location
Nuclear speckles

OverviewNCBI Gene

The protein encoded by this gene is part of a heteromeric complex that binds U4, U5, and U6 small nuclear RNAs and is involved in pre-mRNA splicing. The encoded protein also is a mitotic checkpoint protein and a regulator of chemoresistance in human ovarian cancer. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2016]

Canonical amino-acid sequenceUniProt

522 residues, UniProt reviewed canonical sequence.

>O43172|PRPF4
     1  MASSRASSTQ ATKTKAPDDL VAPVVKKPHI YYGSLEEKER ERLAKGESGI LGKDGLKAGI
    61  EAGNINITSG EVFEIEEHIS ERQAEVLAEF ERRKRARQIN VSTDDSEVKA CLRALGEPIT
   121  LFGEGPAERR ERLRNILSVV GTDALKKTKK DDEKSKKSKE EYQQTWYHEG PNSLKVARLW
   181  IANYSLPRAM KRLEEARLHK EIPETTRTSQ MQELHKSLRS LNNFCSQIGD DRPISYCHFS
   241  PNSKMLATAC WSGLCKLWSV PDCNLLHTLR GHNTNVGAIV FHPKSTVSLD PKDVNLASCA
   301  ADGSVKLWSL DSDEPVADIE GHTVRVARVM WHPSGRFLGT TCYDRSWRLW DLEAQEEILH
   361  QEGHSMGVYD IAFHQDGSLA GTGGLDAFGR VWDLRTGRCI MFLEGHLKEI YGINFSPNGY
   421  HIATGSGDNT CKVWDLRQRR CVYTIPAHQN LVTGVKFEPI HGNFLLTGAY DNTAKIWTHP
   481  GWSPLKTLAG HEGKVMGLDI SSDGQLIATC SYDRTFKLWM AE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PRPF4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.32
Highest tissue expression
24 nTPM

Expression across tissuesHPA

Tissue

  • parathyroid gland: 24 nTPM
  • epididymis: 18 nTPM
  • skeletal muscle: 17 nTPM
  • tonsil: 17 nTPM
  • thymus: 16 nTPM
  • liver: 16 nTPM

Single-cell type

  • neutrophils: 46 nCPM
  • monocyte progenitors: 46 nCPM
  • extravillous trophoblasts: 43 nCPM
  • migrating cytotrophoblasts: 42 nCPM
  • erythrocyte progenitors: 39 nCPM
  • esophageal basal cells: 38 nCPM

Immune cell

  • basophil: 27 nTPM
  • non-classical monocyte: 25 nTPM
  • myeloid DC: 20 nTPM
  • intermediate monocyte: 19 nTPM
  • NK-cell: 18 nTPM
  • naive B-cell: 17 nTPM

Brain region

  • cerebellum: 15 nTPM
  • cerebral cortex: 13 nTPM
  • hypothalamus: 13 nTPM
  • white matter: 13 nTPM
  • basal ganglia: 13 nTPM
  • pons: 13 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about PRPF4.

Disease | AllUniProt

Conditions PRPF4 is implicated in, by any mechanism.

Disease | GeneticClinVar

1 pathogenic / likely-pathogenic of 389 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.23
gnomAD pLI
1
gnomAD missense Z
1.99
DepMap mean gene effect
-1.53
DepMap dependency class
pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of PRPF4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PRPF4 as an antibody target. Whether an autoantibody or antibody against PRPF4 could matter depends on whether native PRPF4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PRPF4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PRPF4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/PRPF4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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