Seroatlas · Human Serome Atlas

PRPF18

Pre-mRNA-splicing factor 18

Also known as: hPrp18, PRP18_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q99633
Gene
PRPF18
Ensembl
ENSG00000165630
Chromosome
10
Canonical length
342 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nuclear speckles,Cytosol

OverviewNCBI Gene

Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to the yeast splicing factor Prp18. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

342 residues, UniProt reviewed canonical sequence.

>Q99633|PRPF18
     1  MDILKSEILR KRQLVEDRNL LVENKKYFKR SELAKKEEEA YFERCGYKIQ PKEEDQKPLT
    61  SSNPVLELEL AEEKLPMTLS RQEVIRRLRE RGEPIRLFGE TDYDAFQRLR KIEILTPEVN
   121  KGLRNDLKAA LDKIDQQYLN EIVGGQEPGE EDTQNDLKVH EENTTIEELE ALGESLGKGD
   181  DHKDMDIITK FLKFLLGVWA KELNAREDYV KRSVQGKLNS ATQKQTESYL RPLFRKLRKR
   241  NLPADIKESI TDIIKFMLQR EYVKANDAYL QMAIGNAPWP IGVTMVGIHA RTGREKIFSK
   301  HVAHVLNDET QRKYIQGLKR LMTICQKHFP TDPSKCVEYN AL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PRPF18 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.38
Highest tissue expression
42 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 42 nTPM
  • epididymis: 28 nTPM
  • thymus: 24 nTPM
  • skeletal muscle: 23 nTPM
  • thyroid gland: 23 nTPM
  • tongue: 21 nTPM

Single-cell type

  • adipocytes: 219 nCPM
  • late spermatids: 188 nCPM
  • cardiomyocytes: 152 nCPM
  • kupffer cells: 143 nCPM
  • neutrophils: 137 nCPM
  • late primary spermatocytes: 137 nCPM

Immune cell

  • neutrophil: 41 nTPM
  • NK-cell: 40 nTPM
  • non-classical monocyte: 38 nTPM
  • eosinophil: 37 nTPM
  • T-reg: 34 nTPM
  • naive CD8 T-cell: 33 nTPM

Brain region

  • cerebellum: 29 nTPM
  • hypothalamus: 25 nTPM
  • white matter: 23 nTPM
  • basal ganglia: 23 nTPM
  • cerebral cortex: 23 nTPM
  • thalamus: 22 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.81
gnomAD pLI
0
gnomAD missense Z
2.55
DepMap mean gene effect
-0.77
DepMap dependency class
common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of PRPF18 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PRPF18 as an antibody target. Whether an autoantibody or antibody against PRPF18 could matter depends on whether native PRPF18 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PRPF18 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PRPF18 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/PRPF18. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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