Seroatlas · Human Serome Atlas

PKN1

Serine/threonine-protein kinase N1

Also known as: DBK, MGC46204, PAK1, PKN, PKN1_HUMAN, PRK1, PRKCL1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q16512
Gene
PKN1
Ensembl
ENSG00000123143
Chromosome
19
Canonical length
942 aa
Protein class
Enzymes, Predicted intracellular proteins
Subcellular location
Plasma membrane,Cytokinetic bridge

OverviewNCBI Gene

The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

942 residues, UniProt reviewed canonical sequence.

>Q16512|PKN1
     1  MASDAVQSEP RSWSLLEQLG LAGADLAAPG VQQQLELERE RLRREIRKEL KLKEGAENLR
    61  RATTDLGRSL GPVELLLRGS SRRLDLLHQQ LQELHAHVVL PDPAATHDGP QSPGAGGPTC
   121  SATNLSRVAG LEKQLAIELK VKQGAENMIQ TYSNGSTKDR KLLLTAQQML QDSKTKIDII
   181  RMQLRRALQA GQLENQAAPD DTQGSPDLGA VELRIEELRH HFRVEHAVAE GAKNVLRLLS
   241  AAKAPDRKAV SEAQEKLTES NQKLGLLREA LERRLGELPA DHPKGRLLRE ELAAASSAAF
   301  STRLAGPFPA THYSTLCKPA PLTGTLEVRV VGCRDLPETI PWNPTPSMGG PGTPDSRPPF
   361  LSRPARGLYS RSGSLSGRSS LKAEAENTSE VSTVLKLDNT VVGQTSWKPC GPNAWDQSFT
   421  LELERARELE LAVFWRDQRG LCALKFLKLE DFLDNERHEV QLDMEPQGCL VAEVTFRNPV
   481  IERIPRLRRQ KKIFSKQQGK AFQRARQMNI DVATWVRLLR RLIPNATGTG TFSPGASPGS
   541  EARTTGDISV EKLNLGTDSD SSPQKSSRDP PSSPSSLSSP IQESTAPELP SETQETPGPA
   601  LCSPLRKSPL TLEDFKFLAV LGRGHFGKVL LSEFRPSGEL FAIKALKKGD IVARDEVESL
   661  MCEKRILAAV TSAGHPFLVN LFGCFQTPEH VCFVMEYSAG GDLMLHIHSD VFSEPRAIFY
   721  SACVVLGLQF LHEHKIVYRD LKLDNLLLDT EGYVKIADFG LCKEGMGYGD RTSTFCGTPE
   781  FLAPEVLTDT SYTRAVDWWG LGVLLYEMLV GESPFPGDDE EEVFDSIVND EVRYPRFLSA
   841  EAIGIMRRLL RRNPERRLGS SERDAEDVKK QPFFRTLGWE ALLARRLPPP FVPTLSGRTD
   901  VSNFDEEFTG EAPTLSPPRD ARPLTAAEQA AFLDFDFVAG GC

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PKN1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.34
Highest tissue expression
86 nTPM

Expression across tissuesHPA

Tissue

  • spleen: 86 nTPM
  • heart muscle: 83 nTPM
  • skeletal muscle: 78 nTPM
  • colon: 69 nTPM
  • lung: 67 nTPM
  • bone marrow: 63 nTPM

Single-cell type

  • hofbauer cells: 114 nCPM
  • nk-cells: 98 nCPM
  • kupffer cells: 95 nCPM
  • alveolar cells type 1: 90 nCPM
  • monocytes: 90 nCPM
  • neutrophil progenitors: 87 nCPM

Immune cell

  • non-classical monocyte: 53 nTPM
  • eosinophil: 41 nTPM
  • intermediate monocyte: 38 nTPM
  • neutrophil: 32 nTPM
  • gdT-cell: 30 nTPM
  • total PBMC: 29 nTPM

Brain region

  • medulla oblongata: 95 nTPM
  • spinal cord: 88 nTPM
  • pons: 88 nTPM
  • cerebral cortex: 88 nTPM
  • basal ganglia: 87 nTPM
  • midbrain: 86 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.39
gnomAD pLI
0.2
gnomAD missense Z
1.34
DepMap mean gene effect
-0.04
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of PKN1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PKN1 as an antibody target. Whether an autoantibody or antibody against PKN1 could matter depends on whether native PKN1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PKN1 is annotated at the cell surface, where native PKN1 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label PKN1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/PKN1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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