Seroatlas · Human Serome Atlas

MAP2K3

Dual specificity mitogen-activated protein kinase kinase 3

Also known as: MAPKK3, MEK3, MKK3, MP2K3_HUMAN, PRKMK3

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P46734
Gene
MAP2K3
Ensembl
ENSG00000034152
Chromosome
17
Canonical length
347 aa
Protein class
Disease related genes, Enzymes, Potential drug targets, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Cytosol

OverviewNCBI Gene

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

347 residues, UniProt reviewed canonical sequence.

>P46734|MAP2K3
     1  MESPASSQPA SMPQSKGKSK RKKDLRISCM SKPPAPNPTP PRNLDSRTFI TIGDRNFEVE
    61  ADDLVTISEL GRGAYGVVEK VRHAQSGTIM AVKRIRATVN SQEQKRLLMD LDINMRTVDC
   121  FYTVTFYGAL FREGDVWICM ELMDTSLDKF YRKVLDKNMT IPEDILGEIA VSIVRALEHL
   181  HSKLSVIHRD VKPSNVLINK EGHVKMCDFG ISGYLVDSVA KTMDAGCKPY MAPERINPEL
   241  NQKGYNVKSD VWSLGITMIE MAILRFPYES WGTPFQQLKQ VVEEPSPQLP ADRFSPEFVD
   301  FTAQCLRKNP AERMSYLELM EHPFFTLHKT KKTDIAAFVK EILGEDS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MAP2K3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.31
Highest tissue expression
200 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 200 nTPM
  • skeletal muscle: 125 nTPM
  • liver: 94 nTPM
  • tongue: 70 nTPM
  • lung: 66 nTPM
  • heart muscle: 63 nTPM

Single-cell type

  • neutrophils: 734 nCPM
  • erythrocytes: 385 nCPM
  • esophageal apical cells: 332 nCPM
  • platelets: 330 nCPM
  • epididymal basal cells: 328 nCPM
  • endometrial glandular cells: 323 nCPM

Immune cell

  • neutrophil: 146 nTPM
  • non-classical monocyte: 105 nTPM
  • eosinophil: 78 nTPM
  • intermediate monocyte: 72 nTPM
  • basophil: 54 nTPM
  • myeloid DC: 50 nTPM

Brain region

  • cerebral cortex: 39 nTPM
  • choroid plexus: 36 nTPM
  • cerebellum: 26 nTPM
  • white matter: 24 nTPM
  • thalamus: 23 nTPM
  • hypothalamus: 21 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.66
gnomAD pLI
0.02
gnomAD missense Z
1.8
DepMap mean gene effect
-0.05
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MAP2K3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MAP2K3 as an antibody target. Whether an autoantibody or antibody against MAP2K3 could matter depends on whether native MAP2K3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MAP2K3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MAP2K3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/MAP2K3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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