MAP3K20

Protein identityUniProt · HPA

UniProt accession

Q9NYL2

Gene

MAP3K20

Ensembl

ENSG00000091436

Chromosome

2

Canonical length

800 aa

Protein class

Disease related genes, Enzymes, Human disease related genes, Potential drug targets, Predicted intracellular proteins

Subcellular location

Cytosol

Quaternary structure

Homodimer

OverviewNCBI Gene

This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

800 residues, UniProt reviewed canonical sequence.

>Q9NYL2|MAP3K20
     1  MSSLGASFVQ IKFDDLQFFE NCGGGSFGSV YRAKWISQDK EVAVKKLLKI EKEAEILSVL
    61  SHRNIIQFYG VILEPPNYGI VTEYASLGSL YDYINSNRSE EMDMDHIMTW ATDVAKGMHY
   121  LHMEAPVKVI HRDLKSRNVV IAADGVLKIC DFGASRFHNH TTHMSLVGTF PWMAPEVIQS
   181  LPVSETCDTY SYGVVLWEML TREVPFKGLE GLQVAWLVVE KNERLTIPSS CPRSFAELLH
   241  QCWEADAKKR PSFKQIISIL ESMSNDTSLP DKCNSFLHNK AEWRCEIEAT LERLKKLERD
   301  LSFKEQELKE RERRLKMWEQ KLTEQSNTPL LPSFEIGAWT EDDVYCWVQQ LVRKGDSSAE
   361  MSVYASLFKE NNITGKRLLL LEEEDLKDMG IVSKGHIIHF KSAIEKLTHD YINLFHFPPL
   421  IKDSGGEPEE NEEKIVNLEL VFGFHLKPGT GPQDCKWKMY MEMDGDEIAI TYIKDVTFNT
   481  NLPDAEILKM TKPPFVMEKW IVGIAKSQTV ECTVTYESDV RTPKSTKHVH SIQWSRTKPQ
   541  DEVKAVQLAI QTLFTNSDGN PGSRSDSSAD CQWLDTLRMR QIASNTSLQR SQSNPILGSP
   601  FFSHFDGQDS YAAAVRRPQV PIKYQQITPV NQSRSSSPTQ YGLTKNFSSL HLNSRDSGFS
   661  SGNTDTSSER GRYSDRSRNK YGRGSISLNS SPRGRYSGKS QHSTPSRGRY PGKFYRVSQS
   721  ALNPHQSPDF KRSPRDLHQP NTIPGMPLHP ETDSRASEED SKVSEGGWTK VEYRKKPHRP
   781  SPAKTNKERA RGDHRGWRNF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MAP3K20 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.43

Highest tissue expression

261 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about MAP3K20.

Disease | AllUniProt

Conditions MAP3K20 is implicated in, by any mechanism.

• Split-foot malformation with mesoaxial polydactyly (SFMMP) MIM:616890
• Myopathy, centronuclear, 6, with fiber-type disproportion (CNM6) MIM:617760

Disease | GeneticClinVar

14 pathogenic / likely-pathogenic of 454 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Split-foot malformation-mesoaxial polydactyly syndrome
• Myopathy, centronuclear, 6, with fiber-type disproportion
• Split hand-foot malformation 1
• Centronuclear myopathy
• MAP3K20-related disorder

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.68

gnomAD pLI

0

DepMap mean gene effect

-0.06

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cell death
• cell differentiation
• cellular response to gamma radiation
• cellular response to UV-B
• chromosome segregation
• cytoskeleton organization
• DNA damage checkpoint signaling
• embryonic digit morphogenesis
• GCN2-mediated signaling
• inflammatory response
• JNK cascade
• limb development
• MAPK cascade
• negative regulation of translation in response to endoplasmic reticulum stress
• p38MAPK cascade
• positive regulation of apoptotic process
• positive regulation of programmed cell death
• protein autophosphorylation
• protein phosphorylation
• pyroptotic inflammatory response
• regulation of mitotic metaphase/anaphase transition
• stress-activated MAPK cascade
• stress-activated protein kinase signaling cascade
• negative regulation of stress-activated protein kinase signaling cascade
• positive regulation of mitotic DNA damage checkpoint

Molecular functions

• ATP binding
• JUN kinase kinase kinase activity
• magnesium ion binding
• MAP kinase kinase kinase activity
• protein kinase activator activity
• protein serine kinase activity
• protein serine/threonine kinase activity
• ribosome binding
• RNA binding
• small ribosomal subunit rRNA binding
• stalled ribosome sensor activity

Cellular components

• cytoplasm
• cytosol
• nucleus

Protein domainsUniProt · Pfam · InterPro

• Protein kinase domain
• Serine-threonine/tyrosine-protein kinase, catalytic domain
• Sterile alpha motif domain
• Serine/threonine-protein kinase, active site
• Protein kinase-like domain superfamily
• Sterile alpha motif/pointed domain superfamily
• Serine/Threonine Kinases and Pseudokinases
• SAM domain (Sterile alpha motif)
• Protein tyrosine and serine/threonine kinase

KeywordsUniProt

• Acetylation
• ATP-binding
• Cell cycle
• Cytoplasm
• Kinase
• Magnesium
• Metal-binding
• Nucleotide-binding
• Nucleus
• Phosphoprotein
• RNA-binding
• rRNA-binding
• Serine/threonine-protein kinase
• Transferase

InteractionsUniProt · HPA

Protein binding partners of MAP3K20 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• PKN1
• ZNF33A
• EIF2AK4
• RPS6KA5
• SFN
• YWHAE
• YWHAG
• YWHAZ