Seroatlas · Human Serome Atlas

PDPK1

3-phosphoinositide-dependent protein kinase 1

Also known as: PDK1, PDPK1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O15530
Gene: PDPK1
Ensembl: ENSG00000140992
Chromosome: 16
Canonical length: 556 aa
Protein class: Enzymes, Plasma proteins, Predicted intracellular proteins
Subcellular location: Primary cilium tip,Cytosol
Quaternary structure: Homodimer

OverviewNCBI Gene

Enables 3-phosphoinositide-dependent protein kinase activity; phospholipase activator activity; and phospholipase binding activity. Involved in several processes, including cell surface receptor signaling pathway; intracellular signaling cassette; and regulation of signal transduction. Acts upstream of or within intracellular signal transduction. Located in cell projection; cytosol; and plasma membrane. Implicated in prostate cancer. Biomarker of lung non-small cell carcinoma. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

556 residues, UniProt reviewed canonical sequence.

>O15530|PDPK1
     1  MARTTSQLYD AVPIQSSVVL CSCPSPSMVR TQTESSTPPG IPGGSRQGPA MDGTAAEPRP
    61  GAGSLQHAQP PPQPRKKRPE DFKFGKILGE GSFSTVVLAR ELATSREYAI KILEKRHIIK
   121  ENKVPYVTRE RDVMSRLDHP FFVKLYFTFQ DDEKLYFGLS YAKNGELLKY IRKIGSFDET
   181  CTRFYTAEIV SALEYLHGKG IIHRDLKPEN ILLNEDMHIQ ITDFGTAKVL SPESKQARAN
   241  SFVGTAQYVS PELLTEKSAC KSSDLWALGC IIYQLVAGLP PFRAGNEYLI FQKIIKLEYD
   301  FPEKFFPKAR DLVEKLLVLD ATKRLGCEEM EGYGPLKAHP FFESVTWENL HQQTPPKLTA
   361  YLPAMSEDDE DCYGNYDNLL SQFGCMQVSS SSSSHSLSAS DTGLPQRSGS NIEQYIHDLD
   421  SNSFELDLQF SEDEKRLLLE KQAGGNPWHQ FVENNLILKM GPVDKRKGLF ARRRQLLLTE
   481  GPHLYYVDPV NKVLKGEIPW SQELRPEAKN FKTFFVHTPN RTYYLMDPSG NAHKWCRKIQ
   541  EVWRQRYQSH PDAAVQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PDPK1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Cell surface
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.34
Highest tissue expression: 26 nTPM

Expression across tissuesHPA

Tissue

parathyroid gland: 26 nTPM
cerebellum: 24 nTPM
basal ganglia: 21 nTPM
cerebral cortex: 19 nTPM
small intestine: 18 nTPM
pancreas: 18 nTPM

Single-cell type

oocytes: 184 nCPM
proximal tubule cells: 157 nCPM
syncytiotrophoblasts: 140 nCPM
neutrophils: 112 nCPM
renal collecting duct intercalated cells: 102 nCPM
renal connecting tubule cells: 93 nCPM

Immune cell

neutrophil: 13 nTPM
non-classical monocyte: 8.8 nTPM
intermediate monocyte: 8.3 nTPM
eosinophil: 8.2 nTPM
plasmacytoid DC: 7.8 nTPM
classical monocyte: 7 nTPM

Brain region

basal ganglia: 88 nTPM
cerebral cortex: 78 nTPM
hippocampal formation: 77 nTPM
amygdala: 68 nTPM
white matter: 61 nTPM
pons: 60 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.29
gnomAD pLI: 0.98
gnomAD missense Z: 2.01
DepMap mean gene effect: -1.04
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

ATP binding
phospholipase activator activity
phospholipase binding
protein serine kinase activity
protein serine/threonine kinase activity
3-phosphoinositide-dependent protein kinase activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of PDPK1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PDPK1 as an antibody target. Whether an autoantibody or antibody against PDPK1 could matter depends on whether native PDPK1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PDPK1 is annotated at the cell surface, where native PDPK1 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label PDPK1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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