GRB14

Protein identityUniProt · HPA

UniProt accession

Q14449

Gene

GRB14

Ensembl

ENSG00000115290

Chromosome

2

Canonical length

540 aa

Protein class

Predicted intracellular proteins

Subcellular location

Nucleoplasm,Cytosol

OverviewNCBI Gene

The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. This protein likely has an inhibitory effect on receptor tyrosine kinase signaling and, in particular, on insulin receptor signaling. This gene may play a role in signaling pathways that regulate growth and metabolism. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Canonical amino-acid sequenceUniProt

540 residues, UniProt reviewed canonical sequence.

>Q14449|GRB14
     1  MTTSLQDGQS AASRAAARDS PLAAQVCGAA QGRGDAHDLA PAPWLHARAL LPLPDGTRGC
    61  AADRRKKKDL DVPEMPSIPN PFPELCCSPF TSVLSADLFP KANSRKKQVI KVYSEDETSR
   121  ALDVPSDITA RDVCQLLILK NHYIDDHSWT LFEHLPHIGV ERTIEDHELV IEVLSNWGIE
   181  EENKLYFRKN YAKYEFFKNP MYFFPEHMVS FATETNGEIS PTQILQMFLS SSTYPEIHGF
   241  LHAKEQGKKS WKKIYFFLRR SGLYFSTKGT SKEPRHLQFF SEFGNSDIYV SLAGKKKHGA
   301  PTNYGFCFKP NKAGGPRDLK MLCAEEEQSR TCWVTAIRLL KYGMQLYQNY MHPYQGRSGC
   361  SSQSISPMRS ISENSLVAMD FSGQKSRVIE NPTEALSVAV EEGLAWRKKG CLRLGTHGSP
   421  TASSQSSATN MAIHRSQPWF HHKISRDEAQ RLIIQQGLVD GVFLVRDSQS NPKTFVLSMS
   481  HGQKIKHFQI IPVEDDGEMF HTLDDGHTRF TDLIQLVEFY QLNKGVLPCK LKHYCARIAL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against GRB14 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.39

Highest tissue expression

43 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.5

gnomAD pLI

0.05

gnomAD missense Z

-0.04

DepMap mean gene effect

-0.12

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• insulin receptor signaling pathway
• negative regulation of insulin receptor signaling pathway
• signal transduction

Molecular functions

• molecular adaptor activity
• protein-macromolecule adaptor activity
• receptor tyrosine kinase binding

Cellular components

• cytoplasm
• cytosol
• endosome membrane
• plasma membrane

Protein domainsUniProt · Pfam · InterPro

• Ras-associating domain
• SH2 domain
• Pleckstrin homology domain
• PH-like domain superfamily
• BPS (Between PH and SH2) domain
• Ubiquitin-like domain superfamily
• SH2 domain superfamily
• GRB/APBB1IP
• APBB1IP, PH domain
• SH2 domain
• PH domain
• BPS (Between PH and SH2)
• RA like domain
• Grb14, SH2 domain

KeywordsUniProt

• Acetylation
• Cytoplasm
• Endosome
• Membrane
• Phosphoprotein
• SH2 domain

InteractionsUniProt · HPA

Protein binding partners of GRB14 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• PDPK1
• TEK