Seroatlas · Human Serome Atlas

EWSR1

RNA-binding protein EWS

Also known as: EWS, EWS_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q01844
Gene
EWSR1
Ensembl
ENSG00000182944
Chromosome
22
Canonical length
656 aa
Protein class
Cancer-related genes, Disease related genes, Plasma proteins, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Nucleoli

OverviewNCBI Gene

This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14. [provided by RefSeq, Jul 2009]

Canonical amino-acid sequenceUniProt

656 residues, UniProt reviewed canonical sequence.

>Q01844|EWSR1
     1  MASTDYSTYS QAAAQQGYSA YTAQPTQGYA QTTQAYGQQS YGTYGQPTDV SYTQAQTTAT
    61  YGQTAYATSY GQPPTGYTTP TAPQAYSQPV QGYGTGAYDT TTATVTTTQA SYAAQSAYGT
   121  QPAYPAYGQQ PAATAPTRPQ DGNKPTETSQ PQSSTGGYNQ PSLGYGQSNY SYPQVPGSYP
   181  MQPVTAPPSY PPTSYSSTQP TSYDQSSYSQ QNTYGQPSSY GQQSSYGQQS SYGQQPPTSY
   241  PPQTGSYSQA PSQYSQQSSS YGQQSSFRQD HPSSMGVYGQ ESGGFSGPGE NRSMSGPDNR
   301  GRGRGGFDRG GMSRGGRGGG RGGMGSAGER GGFNKPGGPM DEGPDLDLGP PVDPDEDSDN
   361  SAIYVQGLND SVTLDDLADF FKQCGVVKMN KRTGQPMIHI YLDKETGKPK GDATVSYEDP
   421  PTAKAAVEWF DGKDFQGSKL KVSLARKKPP MNSMRGGLPP REGRGMPPPL RGGPGGPGGP
   481  GGPMGRMGGR GGDRGGFPPR GPRGSRGNPS GGGNVQHRAG DWQCPNPGCG NQNFAWRTEC
   541  NQCKAPKPEG FLPPPFPPPG GDRGRGGPGG MRGGRGGLMD RGGPGGMFRG GRGGDRGGFR
   601  GGRGMDRGGF GGGRRGGPGG PPGPLMEQMG GRRGGRGGPG KMDKGEHRQE RRDRPY

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EWSR1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.66
Highest tissue expression
170 nTPM

Expression across tissuesHPA

Tissue

  • thymus: 170 nTPM
  • bone marrow: 163 nTPM
  • skeletal muscle: 128 nTPM
  • testis: 125 nTPM
  • tonsil: 118 nTPM
  • lymph node: 112 nTPM

Single-cell type

  • late primary spermatocytes: 357 nCPM
  • syncytiotrophoblasts: 199 nCPM
  • tuft cells: 173 nCPM
  • cytotrophoblasts: 171 nCPM
  • neutrophils: 170 nCPM
  • extravillous trophoblasts: 169 nCPM

Immune cell

  • total PBMC: 314 nTPM
  • non-classical monocyte: 295 nTPM
  • intermediate monocyte: 284 nTPM
  • T-reg: 245 nTPM
  • myeloid DC: 228 nTPM
  • NK-cell: 218 nTPM

Brain region

  • cerebral cortex: 148 nTPM
  • white matter: 124 nTPM
  • cerebellum: 123 nTPM
  • medulla oblongata: 120 nTPM
  • midbrain: 115 nTPM
  • hippocampal formation: 113 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about EWSR1.

Disease | AllUniProt

Conditions EWSR1 is implicated in, by any mechanism.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.22
gnomAD pLI
1
gnomAD missense Z
2.52
DepMap mean gene effect
-0.86
DepMap dependency class
common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of EWSR1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads EWSR1 as an antibody target. Whether an autoantibody or antibody against EWSR1 could matter depends on whether native EWSR1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

EWSR1 is annotated at the cell surface, where native EWSR1 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label EWSR1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/EWSR1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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