Seroatlas · Human Serome Atlas

EIF4A1

Eukaryotic initiation factor 4A-I

Also known as: DDX2A, EIF-4A, EIF4A, IF4A1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P60842
Gene
EIF4A1
Ensembl
ENSG00000161960
Chromosome
17
Canonical length
406 aa
Protein class
Enzymes, Plasma proteins, Predicted intracellular proteins
Subcellular location
Cytosol

OverviewNCBI Gene

Enables double-stranded RNA binding activity and translation initiation factor activity. Involved in cytoplasmic translational initiation and positive regulation of transcription by RNA polymerase II. Located in nuclear stress granule; perinuclear region of cytoplasm; and plasma membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

406 residues, UniProt reviewed canonical sequence.

>P60842|EIF4A1
     1  MSASQDSRSR DNGPDGMEPE GVIESNWNEI VDSFDDMNLS ESLLRGIYAY GFEKPSAIQQ
    61  RAILPCIKGY DVIAQAQSGT GKTATFAISI LQQIELDLKA TQALVLAPTR ELAQQIQKVV
   121  MALGDYMGAS CHACIGGTNV RAEVQKLQME APHIIVGTPG RVFDMLNRRY LSPKYIKMFV
   181  LDEADEMLSR GFKDQIYDIF QKLNSNTQVV LLSATMPSDV LEVTKKFMRD PIRILVKKEE
   241  LTLEGIRQFY INVEREEWKL DTLCDLYETL TITQAVIFIN TRRKVDWLTE KMHARDFTVS
   301  AMHGDMDQKE RDVIMREFRS GSSRVLITTD LLARGIDVQQ VSLVINYDLP TNRENYIHRI
   361  GRGGRFGRKG VAINMVTEED KRTLRDIETF YNTSIEEMPL NVADLI

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EIF4A1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.25
Highest tissue expression
532 nTPM

Expression across tissuesHPA

Tissue

  • esophagus: 532 nTPM
  • urinary bladder: 472 nTPM
  • adipose tissue: 466 nTPM
  • bone marrow: 379 nTPM
  • tonsil: 360 nTPM
  • skin: 353 nTPM

Single-cell type

  • microglia: 82 nCPM
  • oligodendrocytes: 54 nCPM
  • other brain neurons: 54 nCPM
  • astrocytes: 52 nCPM
  • bergmann glia: 50 nCPM
  • oligodendrocyte progenitor cells: 48 nCPM

Immune cell

  • total PBMC: 2,954 nTPM
  • myeloid DC: 1,910 nTPM
  • classical monocyte: 1,645 nTPM
  • intermediate monocyte: 1,382 nTPM
  • non-classical monocyte: 1,083 nTPM
  • MAIT T-cell: 756 nTPM

Brain region

  • thalamus: 129 nTPM
  • white matter: 126 nTPM
  • medulla oblongata: 122 nTPM
  • hypothalamus: 110 nTPM
  • spinal cord: 109 nTPM
  • pons: 108 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.12
gnomAD pLI
1
gnomAD missense Z
3.93
DepMap mean gene effect
-1.25
DepMap dependency class
common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of EIF4A1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads EIF4A1 as an antibody target. Whether an autoantibody or antibody against EIF4A1 could matter depends on whether native EIF4A1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

EIF4A1 is annotated at the cell surface, where native EIF4A1 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label EIF4A1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/EIF4A1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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