BMI1

Protein identityUniProt · HPA

UniProt accession

P35226

Gene

BMI1

Ensembl

ENSG00000168283

Chromosome

10

Canonical length

326 aa

Protein class

Cancer-related genes, Human disease related genes, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Nucleoli,Nuclear bodies,Cytosol

OverviewNCBI Gene

This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene. [provided by RefSeq, Sep 2015]

Canonical amino-acid sequenceUniProt

326 residues, UniProt reviewed canonical sequence.

>P35226|BMI1
     1  MHRTTRIKIT ELNPHLMCVL CGGYFIDATT IIECLHSFCK TCIVRYLETS KYCPICDVQV
    61  HKTRPLLNIR SDKTLQDIVY KLVPGLFKNE MKRRRDFYAA HPSADAANGS NEDRGEVADE
   121  DKRIITDDEI ISLSIEFFDQ NRLDRKVNKD KEKSKEEVND KRYLRCPAAM TVMHLRKFLR
   181  SKMDIPNTFQ IDVMYEEEPL KDYYTLMDIA YIYTWRRNGP LPLKYRVRPT CKRMKISHQR
   241  DGLTNAGELE SDSGSDKANS PAGGIPSTSS CLPSPSTPVQ SPHPQFPHIS STMNGTSNSP
   301  SGNHQSSFAN RPRKSSVNGS SATSSG

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against BMI1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.47

Highest tissue expression

54 nTPM

Expression across tissuesHPA

ReferencesPubMed · IEDB

Publications for BMI1 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.35

gnomAD pLI

0.94

gnomAD missense Z

2.43

DepMap mean gene effect

0.11

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• apoptotic signaling pathway
• brain development
• cellular response to dexamethasone stimulus
• cellular response to interleukin-1
• chromatin remodeling
• DNA methylation-dependent constitutive heterochromatin formation
• embryonic skeletal system morphogenesis
• hemopoiesis
• humoral immune response
• in utero embryonic development
• negative regulation of apoptotic signaling pathway
• negative regulation of gene expression, epigenetic
• negative regulation of transcription by RNA polymerase II
• positive regulation of B cell proliferation
• positive regulation of fibroblast proliferation
• positive regulation of immature T cell proliferation in thymus
• positive regulation of ubiquitin-protein transferase activity
• regulation of adaxial/abaxial pattern formation
• regulation of gene expression
• regulation of kidney development
• rostrocaudal neural tube patterning
• segment specification
• somatic stem cell division

Molecular functions

• promoter-specific chromatin binding
• RING-like zinc finger domain binding
• ubiquitin-protein transferase activator activity
• zinc ion binding

Cellular components

• cytosol
• heterochromatin
• nuclear body
• nucleoplasm
• nucleus
• PcG protein complex
• PRC1 complex
• ubiquitin ligase complex

Protein domainsUniProt · Pfam · InterPro

• Zinc finger, RING-type
• Zinc finger, RING/FYVE/PHD-type
• Zinc finger, RING-type, conserved site
• RAWUL domain
• Zinc finger, C3HC4 type (RING finger)
• RAWUL domain RING finger- and WD40-associated ubiquitin-like

KeywordsUniProt

• Chromatin regulator
• Cytoplasm
• Metal-binding
• Nucleus
• Proto-oncogene
• Repressor
• Transcription
• Transcription regulation
• Ubl conjugation
• Zinc
• Zinc-finger

InteractionsUniProt · HPA

Protein binding partners of BMI1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• CBX7
• CBX8
• E4F1
• PHC2
• RING1
• SPOP
• PHC1
• UBAP2L
• CBX6
• RNF2
• CBX4
• CSNK2A1
• PRPF6
• RYBP
• USP11
• USP7
• YAF2