Seroatlas · Human Serome Atlas

SPOP

Speckle-type POZ protein

Also known as: BTBD32, SPOP_HUMAN, TEF2

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O43791
Gene
SPOP
Ensembl
ENSG00000121067
Chromosome
17
Canonical length
374 aa
Protein class
Cancer-related genes, Disease related genes, Human disease related genes, Predicted intracellular proteins
Quaternary structure
Homodimer

OverviewNCBI Gene

This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

374 residues, UniProt reviewed canonical sequence.

>O43791|SPOP
     1  MSRVPSPPPP AEMSSGPVAE SWCYTQIKVV KFSYMWTINN FSFCREEMGE VIKSSTFSSG
    61  ANDKLKWCLR VNPKGLDEES KDYLSLYLLL VSCPKSEVRA KFKFSILNAK GEETKAMESQ
   121  RAYRFVQGKD WGFKKFIRRD FLLDEANGLL PDDKLTLFCE VSVVQDSVNI SGQNTMNMVK
   181  VPECRLADEL GGLWENSRFT DCCLCVAGQE FQAHKAILAA RSPVFSAMFE HEMEESKKNR
   241  VEINDVEPEV FKEMMCFIYT GKAPNLDKMA DDLLAAADKY ALERLKVMCE DALCSNLSVE
   301  NAAEILILAD LHSADQLKTQ AVDFINYHAS DVLETSGWKS MVVSHPHLVA EAYRSLASAQ
   361  CPFLGPPRKR LKQS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SPOP can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.33
Highest tissue expression
81 nTPM

Expression across tissuesHPA

Tissue

  • colon: 81 nTPM
  • seminal vesicle: 77 nTPM
  • smooth muscle: 73 nTPM
  • blood vessel: 63 nTPM
  • prostate: 61 nTPM
  • skeletal muscle: 53 nTPM

Single-cell type

  • myonuclei: 313 nCPM
  • alveolar cells type 1: 229 nCPM
  • choroid plexus epithelial cells: 193 nCPM
  • neutrophils: 189 nCPM
  • smooth muscle cells: 188 nCPM
  • pituicytes/fscs: 172 nCPM

Immune cell

  • basophil: 145 nTPM
  • neutrophil: 108 nTPM
  • naive CD4 T-cell: 98 nTPM
  • eosinophil: 93 nTPM
  • total PBMC: 92 nTPM
  • myeloid DC: 92 nTPM

Brain region

  • cerebellum: 91 nTPM
  • white matter: 77 nTPM
  • hypothalamus: 68 nTPM
  • basal ganglia: 66 nTPM
  • choroid plexus: 65 nTPM
  • hippocampal formation: 63 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about SPOP.

Disease | AllUniProt

Conditions SPOP is implicated in, by any mechanism.

Disease | GeneticClinVar

12 pathogenic / likely-pathogenic of 243 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

  • Neurodevelopmental disorder with relative macrocephaly and with or without cardiac or endocrine anomalies
  • Neurodevelopmental disorder with microcephaly and dysmorphic facies
  • Inborn genetic diseases
  • SPOP-related neurodevelopmental condition
  • SPOP-related disorder

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.14
gnomAD pLI
1
gnomAD missense Z
4.14
DepMap mean gene effect
-0.1
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of SPOP in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SPOP as an antibody target. Whether an autoantibody or antibody against SPOP could matter depends on whether native SPOP is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SPOP is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label SPOP as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/SPOP. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

© 2026 · Seroatlas, Discover Oregon LLC · Terms of use

Loading the interactive Seroatlas protein explorer...