E4F1
Transcription factor E4F1
Also known as: E4F, E4F1_HUMAN
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- Q66K89
- Gene
- E4F1
- Ensembl
- ENSG00000167967
- Chromosome
- 16
- Canonical length
- 784 aa
- Protein class
- Enzymes, Predicted intracellular proteins, Transcription factors
- Subcellular location
- Nucleoplasm
- Quaternary structure
- Homodimer
OverviewNCBI Gene
The zinc finger protein encoded by this gene is one of several cellular transcription factors whose DNA-binding activities are regulated through the action of adenovirus E1A. A 50-kDa amino-terminal product is generated from the full-length protein through proteolytic cleavage. The protein is differentially regulated by E1A-induced phosphorylation. The full-length gene product represses transcription from the E4 promoter in the absence of E1A, while the 50-kDa form acts as a transcriptional activator in its presence. Alternative splicing results in multiple transcripts encoding different proteins. [provided by RefSeq, Jan 2014]
Canonical amino-acid sequenceUniProt
784 residues, UniProt reviewed canonical sequence.
>Q66K89|E4F1
1 MEGAMAVRVT AAHTAEAQAE AGREAGEGAV AAVAAALAPS GFLGLPAPFS EEDEDDVHRC
61 GRCQAEFTAL EDFVQHKIQK ACQRAPPEAL PATPATTALL GQEVVPAAPG PEEPITVAHI
121 VVEAASLAAD ISHASDLVGG GHIKEVIVAA EAELGDGEMA EAPGSPRQQG LGLAGEGEQA
181 QVKLLVNKDG RYVCALCHKT FKTGSILKAH MVTHSSRKDH ECKLCGASFR TKGSLIRHHR
241 RHTDERPYKC SKCGKSFRES GALTRHLKSL TPCTEKIRFS VSKDVVVSKE DARAGSGAGA
301 AGLGTATSSV TGEPIETSPV IHLVTDAKGT VIHEVHVQMQ ELSLGMKALA PEPPVSQELP
361 CSSEGSRENL LHQAMQNSGI VLERAAGEEG ALEPAPAAGS SPQPLAVAAP QLPVLEVQPL
421 ETQVASEASA VPRTHPCPQC SETFPTAATL EAHKRGHTGP RPFACAQCGK AFPKAYLLKK
481 HQEVHVRERR FRCGDCGKLY KTIAHVRGHR RVHSDERPYP CPKCGKRYKT KNAQQVHFRT
541 HLEEKPHVCQ FCSRGFREKG SLVRHVRHHT GEKPFKCYKC GRGFAEHGTL NRHLRTKGGC
601 LLEVEELLVS EDSPAAATTV LTEDPHTVLV EFSSVVADTQ EYIIEATADD AETSEATEII
661 EGTQTEVDSH IMKVVQQIVH QASAGHQIIV QNVTMDEETA LGPEAAAADT ITIATPESLT
721 EQVAMTLASA ISEGTVLAAR AGTSGTEQAT VTMVSSEDIE ILEHAGELVI ASPEGQLEVQ
781 TVIVLocalizationUniProt · AlphaFold · HPA
Whether an antibody against E4F1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.59
- Highest tissue expression
- 32 nTPM
Expression across tissuesHPA
Tissue
- cerebellum: 32 nTPM
- skin: 23 nTPM
- thyroid gland: 22 nTPM
- prostate: 21 nTPM
- spleen: 20 nTPM
- liver: 19 nTPM
Single-cell type
- cone photoreceptor cells: 35 nCPM
- cytotrophoblasts: 32 nCPM
- colonocytes: 28 nCPM
- goblet cells: 28 nCPM
- myonuclei: 27 nCPM
- migrating cytotrophoblasts: 27 nCPM
Immune cell
- non-classical monocyte: 12 nTPM
- eosinophil: 12 nTPM
- neutrophil: 11 nTPM
- T-reg: 11 nTPM
- gdT-cell: 10 nTPM
- intermediate monocyte: 9.8 nTPM
Brain region
- cerebellum: 25 nTPM
- pons: 25 nTPM
- cerebral cortex: 25 nTPM
- medulla oblongata: 24 nTPM
- white matter: 23 nTPM
- midbrain: 22 nTPM
DiseaseUniProt · ClinVar · IEDB · PubMed
Four sources answering four different questions about E4F1.
Disease | GeneticClinVar
1 pathogenic / likely-pathogenic of 157 ClinVar records.
Conditions with pathogenic or likely-pathogenic variants.
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.48
- gnomAD pLI
- 0.08
- gnomAD missense Z
- 1.35
- DepMap mean gene effect
- -0.43
- DepMap dependency class
- selective
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- cell division
- DNA replication
- negative regulation of transcription by RNA polymerase II
- positive regulation of transcription by RNA polymerase II
- protein ubiquitination
- regulation of cell cycle
- regulation of cell cycle process
- regulation of transcription by RNA polymerase II
- regulation of mitotic cell cycle, embryonic
Molecular functions
- cAMP response element binding
- DNA-binding transcription activator activity, RNA polymerase II-specific
- DNA-binding transcription factor activity
- DNA-binding transcription repressor activity, RNA polymerase II-specific
- RNA polymerase II cis-regulatory region sequence-specific DNA binding
- RNA polymerase II transcription regulatory region sequence-specific DNA binding
- RNA polymerase II-specific DNA-binding transcription factor binding
- ubiquitin protein ligase activity
- zinc ion binding
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of E4F1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads E4F1 as an antibody target. Whether an autoantibody or antibody against E4F1 could matter depends on whether native E4F1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
E4F1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label E4F1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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