ATP5F1B

Protein identityUniProt · HPA

UniProt accession

P06576

Gene

ATP5F1B

Ensembl

ENSG00000110955

Chromosome

12

Canonical length

529 aa

Protein class

Enzymes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins, Transporters

Subcellular location

Mitochondria

Quaternary structure

Homooctamer

OverviewNCBI Gene

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

529 residues, UniProt reviewed canonical sequence.

>P06576|ATP5F1B
     1  MLGFVGRVAA APASGALRRL TPSASLPPAQ LLLRAAPTAV HPVRDYAAQT SPSPKAGAAT
    61  GRIVAVIGAV VDVQFDEGLP PILNALEVQG RETRLVLEVA QHLGESTVRT IAMDGTEGLV
   121  RGQKVLDSGA PIKIPVGPET LGRIMNVIGE PIDERGPIKT KQFAPIHAEA PEFMEMSVEQ
   181  EILVTGIKVV DLLAPYAKGG KIGLFGGAGV GKTVLIMELI NNVAKAHGGY SVFAGVGERT
   241  REGNDLYHEM IESGVINLKD ATSKVALVYG QMNEPPGARA RVALTGLTVA EYFRDQEGQD
   301  VLLFIDNIFR FTQAGSEVSA LLGRIPSAVG YQPTLATDMG TMQERITTTK KGSITSVQAI
   361  YVPADDLTDP APATTFAHLD ATTVLSRAIA ELGIYPAVDP LDSTSRIMDP NIVGSEHYDV
   421  ARGVQKILQD YKSLQDIIAI LGMDELSEED KLTVSRARKI QRFLSQPFQV AEVFTGHMGK
   481  LVPLKETIKG FQQILAGEYD HLPEQAFYMV GPIEEAVAKA DKLAEEHSS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ATP5F1B can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.29

Highest tissue expression

2,993 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about ATP5F1B.

Disease | AllUniProt

Conditions ATP5F1B is implicated in, by any mechanism.

• Hypermetabolism due to uncoupled mitochondrial oxidative phosphorylation 2 (HUMOP2) MIM:620085

Disease | GeneticClinVar

1 pathogenic / likely-pathogenic of 61 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Hypermetabolism due to uncoupled mitochondrial oxidative phosphorylation 2
• Hypermetabolism due to Defect in Mitochondrial Coupling

Disease | ImmuneIEDB

Conditions an epitope on ATP5F1B was assayed in.

• allergic disease T cell
• Chagas disease B cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.31

gnomAD pLI

0.98

DepMap mean gene effect

-0.93

DepMap dependency class

common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• angiogenesis
• ATP biosynthetic process
• cellular response to interleukin-7
• generation of precursor metabolites and energy
• lipid metabolic process
• negative regulation of cell adhesion involved in substrate-bound cell migration
• osteoblast differentiation
• positive regulation of blood vessel endothelial cell migration
• proton motive force-driven ATP synthesis
• proton motive force-driven mitochondrial ATP synthesis
• proton transmembrane transport
• regulation of intracellular pH

Molecular functions

• angiostatin binding
• ATP binding
• metal ion binding
• MHC class I protein binding
• proton-transporting ATP synthase activity, rotational mechanism
• proton-transporting ATPase activity, rotational mechanism

Cellular components

• cell surface
• extracellular exosome
• membrane
• mitochondrial inner membrane
• mitochondrial matrix
• mitochondrial membrane
• mitochondrial nucleoid
• mitochondrion
• nucleus
• plasma membrane
• proton-transporting ATP synthase complex

Protein domainsUniProt · Pfam · InterPro

• ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain
• AAA+ ATPase domain
• ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal domain
• ATPase, alpha/beta subunit, nucleotide-binding domain, active site
• ATPase, F1/V1 complex, beta/alpha subunit, C-terminal
• P-loop containing nucleoside triphosphate hydrolase
• ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal domain superfamily
• ATP synthase A/B type, C-terminal domain
• ATP synthase alpha/beta family, nucleotide-binding domain
• ATP synthase alpha/beta family, beta-barrel domain
• C-terminal domain of V and A type ATP synthase
• ATP synthase, F1 complex, beta subunit
• ATPase alpha/beta chains

KeywordsUniProt

• Acetylation
• ATP synthesis
• ATP-binding
• CF(1)
• Glycoprotein
• Hydrogen ion transport
• Ion transport
• Magnesium
• Membrane
• Metal-binding
• Mitochondrion
• Mitochondrion inner membrane
• Nucleotide-binding
• Phosphoprotein
• Transit peptide
• Translocase
• Transport

InteractionsUniProt · HPA

Protein binding partners of ATP5F1B in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• CLN5
• MTLN
• PPIF
• PPT1
• S100A1
• ATPAF1
• BCL2L1
• TTC5
• ATPAF2
• ATP5F1A
• ATP5F1C
• ATP5IF1
• ATP5PB
• ATP5PD
• ATP5PF
• LNX2
• SLC35F1