Seroatlas · Human Serome Atlas

ATPAF1

ATP synthase mitochondrial F1 complex assembly factor 1

Also known as: ATP11, Atp11p, ATPF1_HUMAN, FLJ22351

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q5TC12
Gene
ATPAF1
Ensembl
ENSG00000123472
Chromosome
1
Canonical length
328 aa
Protein class
Predicted intracellular proteins
Subcellular location
Mitochondria

OverviewNCBI Gene

This gene encodes an assembly factor for the F(1) component of the mitochondrial ATP synthase. This protein binds specifically to the F1 beta subunit and is thought to prevent this subunit from forming nonproductive homooligomers during enzyme assembly. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Aug 2011]

Canonical amino-acid sequenceUniProt

328 residues, UniProt reviewed canonical sequence.

>Q5TC12|ATPAF1
     1  MAAVVVAAAG GAGPAVLQVA GLYRGLCAVR SRALGLGLVS PAQLRVFPVR PGSGRPEGGA
    61  DSSGVGAEAE LQANPFYDRY RDKIQLLRRS DPAAFESRLE KRSEFRKQPV GHSRQGDFIK
   121  CVEQKTDALG KQSVNRGFTK DKTLSSIFNI EMVKEKTAEE IKQIWQQYFA AKDTVYAVIP
   181  AEKFDLIWNR AQSCPTFLCA LPRREGYEFF VGQWTGTELH FTALINIQTR GEAAASQLIL
   241  YHYPELKEEK GIVLMTAEMD STFLNVAEAQ CIANQVQLFY ATDRKETYGL VETFNLRPNE
   301  FKYMSVIAEL EQSGLGAELK CAQNQNKT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ATPAF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Other membrane
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.4
Highest tissue expression
296 nTPM

Expression across tissuesHPA

Tissue

  • tongue: 296 nTPM
  • skeletal muscle: 260 nTPM
  • heart muscle: 249 nTPM
  • choroid plexus: 168 nTPM
  • parathyroid gland: 130 nTPM
  • kidney: 107 nTPM

Single-cell type

  • late spermatids: 647 nCPM
  • early spermatids: 371 nCPM
  • parietal cells: 126 nCPM
  • choroid plexus epithelial cells: 94 nCPM
  • cytotrophoblasts: 88 nCPM
  • early primary spermatocytes: 82 nCPM

Immune cell

  • NK-cell: 5.7 nTPM
  • T-reg: 5 nTPM
  • eosinophil: 4.8 nTPM
  • naive CD4 T-cell: 3.6 nTPM
  • intermediate monocyte: 3.4 nTPM
  • MAIT T-cell: 3.2 nTPM

Brain region

  • choroid plexus: 146 nTPM
  • hypothalamus: 142 nTPM
  • cerebellum: 121 nTPM
  • thalamus: 115 nTPM
  • basal ganglia: 114 nTPM
  • amygdala: 102 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.72
gnomAD pLI
0.01
gnomAD missense Z
1.18
DepMap mean gene effect
0.02
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • ATP11
  • ATP11 protein

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ATPAF1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ATPAF1 as an antibody target. Whether an autoantibody or antibody against ATPAF1 could matter depends on whether native ATPAF1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ATPAF1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ATPAF1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ATPAF1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

© 2026 · Seroatlas, Discover Oregon LLC · Terms of use

Loading the interactive Seroatlas protein explorer...