PPIF
Peptidyl-prolyl cis-trans isomerase F, mitochondrial
Also known as: Cyp-D, CypD, hCyP3, PPIF_HUMAN
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- P30405
- Gene
- PPIF
- Ensembl
- ENSG00000108179
- Chromosome
- 10
- Canonical length
- 207 aa
- Protein class
- Enzymes, FDA approved drug targets, Metabolic proteins, Plasma proteins, Predicted intracellular proteins, Transporters
- Subcellular location
- Mitochondria
OverviewNCBI Gene
The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. [provided by RefSeq, Jul 2008]
Canonical amino-acid sequenceUniProt
207 residues, UniProt reviewed canonical sequence.
>P30405|PPIF
1 MLALRCGSRW LGLLSVPRSV PLRLPAARAC SKGSGDPSSS SSSGNPLVYL DVDANGKPLG
61 RVVLELKADV VPKTAENFRA LCTGEKGFGY KGSTFHRVIP SFMCQAGDFT NHNGTGGKSI
121 YGSRFPDENF TLKHVGPGVL SMANAGPNTN GSQFFICTIK TDWLDGKHVV FGHVKEGMDV
181 VKKIESFGSK SGRTSKKIVI TDCGQLSLocalizationUniProt · AlphaFold · HPA
Whether an antibody against PPIF can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.35
- Highest tissue expression
- 215 nTPM
Expression across tissuesHPA
Tissue
- adrenal gland: 215 nTPM
- liver: 170 nTPM
- bone marrow: 159 nTPM
- tongue: 125 nTPM
- choroid plexus: 116 nTPM
- heart muscle: 111 nTPM
Single-cell type
- megakaryocytes: 773 nCPM
- esophageal apical cells: 329 nCPM
- monocytes: 308 nCPM
- megakaryocyte progenitors: 239 nCPM
- neutrophils: 207 nCPM
- cdc: 178 nCPM
Immune cell
- neutrophil: 75 nTPM
- myeloid DC: 43 nTPM
- classical monocyte: 34 nTPM
- intermediate monocyte: 28 nTPM
- memory B-cell: 19 nTPM
- eosinophil: 18 nTPM
Brain region
- cerebral cortex: 112 nTPM
- choroid plexus: 96 nTPM
- white matter: 90 nTPM
- cerebellum: 84 nTPM
- thalamus: 82 nTPM
- midbrain: 80 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 1.35
- gnomAD pLI
- 0
- gnomAD missense Z
- 0.68
- DepMap mean gene effect
- -0.13
- DepMap dependency class
- selective
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- apoptotic mitochondrial changes
- cellular response to arsenic-containing substance
- cellular response to calcium ion
- cellular response to hydrogen peroxide
- mitochondrial depolarization
- mitochondrial outer membrane permeabilization involved in programmed cell death
- necroptotic process
- negative regulation of apoptotic process
- negative regulation of intrinsic apoptotic signaling pathway
- negative regulation of oxidative phosphorylation
- negative regulation of release of cytochrome c from mitochondria
- protein folding
- regulation of mitochondrial membrane permeability
- regulation of mitochondrial membrane permeability involved in programmed necrotic cell death
- response to ischemia
- skeletal muscle fiber differentiation
- regulation of proton-transporting ATPase activity, rotational mechanism
Molecular functions
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of PPIF in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads PPIF as an antibody target. Whether an autoantibody or antibody against PPIF could matter depends on whether native PPIF is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
PPIF is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label PPIF as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
Loading the interactive Seroatlas protein explorer...