Seroatlas · Human Serome Atlas

AGO3

Protein argonaute-3

Also known as: AGO3_HUMAN, EIF2C3, FLJ12765, hAGO3

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9H9G7
Gene
AGO3
Ensembl
ENSG00000126070
Chromosome
1
Canonical length
860 aa
Protein class
Enzymes, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Cytosol,Cytoplasmic bodies

OverviewNCBI Gene

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, contains a PAZ domain and a PIWI domain, and may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a tandem cluster of closely related family members including argonaute 4 and eukaryotic translation initiation factor 2C, 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

860 residues, UniProt reviewed canonical sequence.

>Q9H9G7|AGO3
     1  MEIGSAGPAG AQPLLMVPRR PGYGTMGKPI KLLANCFQVE IPKIDVYLYE VDIKPDKCPR
    61  RVNREVVDSM VQHFKVTIFG DRRPVYDGKR SLYTANPLPV ATTGVDLDVT LPGEGGKDRP
   121  FKVSIKFVSR VSWHLLHEVL TGRTLPEPLE LDKPISTNPV HAVDVVLRHL PSMKYTPVGR
   181  SFFSAPEGYD HPLGGGREVW FGFHQSVRPA MWKMMLNIDV SATAFYKAQP VIQFMCEVLD
   241  IHNIDEQPRP LTDSHRVKFT KEIKGLKVEV THCGTMRRKY RVCNVTRRPA SHQTFPLQLE
   301  NGQTVERTVA QYFREKYTLQ LKYPHLPCLQ VGQEQKHTYL PLEVCNIVAG QRCIKKLTDN
   361  QTSTMIKATA RSAPDRQEEI SRLVRSANYE TDPFVQEFQF KVRDEMAHVT GRVLPAPMLQ
   421  YGGRNRTVAT PSHGVWDMRG KQFHTGVEIK MWAIACFATQ RQCREEILKG FTDQLRKISK
   481  DAGMPIQGQP CFCKYAQGAD SVEPMFRHLK NTYSGLQLII VILPGKTPVY AEVKRVGDTL
   541  LGMATQCVQV KNVIKTSPQT LSNLCLKINV KLGGINNILV PHQRPSVFQQ PVIFLGADVT
   601  HPPAGDGKKP SIAAVVGSMD AHPSRYCATV RVQRPRQEII QDLASMVREL LIQFYKSTRF
   661  KPTRIIFYRD GVSEGQFRQV LYYELLAIRE ACISLEKDYQ PGITYIVVQK RHHTRLFCAD
   721  RTERVGRSGN IPAGTTVDTD ITHPYEFDFY LCSHAGIQGT SRPSHYHVLW DDNCFTADEL
   781  QLLTYQLCHT YVRCTRSVSI PAPAYYAHLV AFRARYHLVD KEHDSAEGSH VSGQSNGRDP
   841  QALAKAVQIH QDTLRTMYFA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against AGO3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.25
Highest tissue expression
7 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 7 nTPM
  • pituitary gland: 5.7 nTPM
  • testis: 5.2 nTPM
  • retina: 4.7 nTPM
  • tongue: 4.1 nTPM
  • cerebellum: 3.8 nTPM

Single-cell type

  • somatotrophs: 575 nCPM
  • neutrophil progenitors: 465 nCPM
  • neutrophils: 441 nCPM
  • lactotrophs: 437 nCPM
  • thyrotrophs: 409 nCPM
  • choroid plexus epithelial cells: 316 nCPM

Immune cell

  • eosinophil: 13 nTPM
  • neutrophil: 13 nTPM
  • basophil: 9.9 nTPM
  • memory B-cell: 6.5 nTPM
  • naive B-cell: 6.4 nTPM
  • non-classical monocyte: 6.2 nTPM

Brain region

  • white matter: 41 nTPM
  • medulla oblongata: 32 nTPM
  • thalamus: 31 nTPM
  • basal ganglia: 31 nTPM
  • cerebral cortex: 30 nTPM
  • pons: 30 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.19
gnomAD pLI
1
gnomAD missense Z
5.26
DepMap mean gene effect
-0.2
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of AGO3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads AGO3 as an antibody target. Whether an autoantibody or antibody against AGO3 could matter depends on whether native AGO3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

AGO3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label AGO3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/AGO3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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