Seroatlas · Human Serome Atlas

AGO4

Protein argonaute-4

Also known as: AGO4_HUMAN, EIF2C4, FLJ20033, hAGO4, KIAA1567

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9HCK5
Gene: AGO4
Ensembl: ENSG00000134698
Chromosome: 1
Canonical length: 861 aa
Protein class: Predicted intracellular proteins
Subcellular location: Cytosol,Cytoplasmic bodies

OverviewNCBI Gene

This gene encodes a member of the Argonaute family of proteins which contain PAZ and PIWI domains and play an integral role in RNA interference and short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a cluster of related family members. [provided by RefSeq, Mar 2017]

Canonical amino-acid sequenceUniProt

861 residues, UniProt reviewed canonical sequence.

>Q9HCK5|AGO4
     1  MEALGPGPPA SLFQPPRRPG LGTVGKPIRL LANHFQVQIP KIDVYHYDVD IKPEKRPRRV
    61  NREVVDTMVR HFKMQIFGDR QPGYDGKRNM YTAHPLPIGR DRVDMEVTLP GEGKDQTFKV
   121  SVQWVSVVSL QLLLEALAGH LNEVPDDSVQ ALDVITRHLP SMRYTPVGRS FFSPPEGYYH
   181  PLGGGREVWF GFHQSVRPAM WNMMLNIDVS ATAFYRAQPI IEFMCEVLDI QNINEQTKPL
   241  TDSQRVKFTK EIRGLKVEVT HCGQMKRKYR VCNVTRRPAS HQTFPLQLEN GQAMECTVAQ
   301  YFKQKYSLQL KYPHLPCLQV GQEQKHTYLP LEVCNIVAGQ RCIKKLTDNQ TSTMIKATAR
   361  SAPDRQEEIS RLVKSNSMVG GPDPYLKEFG IVVHNEMTEL TGRVLPAPML QYGGRNKTVA
   421  TPNQGVWDMR GKQFYAGIEI KVWAVACFAP QKQCREDLLK SFTDQLRKIS KDAGMPIQGQ
   481  PCFCKYAQGA DSVEPMFKHL KMTYVGLQLI VVILPGKTPV YAEVKRVGDT LLGMATQCVQ
   541  VKNVVKTSPQ TLSNLCLKIN AKLGGINNVL VPHQRPSVFQ QPVIFLGADV THPPAGDGKK
   601  PSIAAVVGSM DGHPSRYCAT VRVQTSRQEI SQELLYSQEV IQDLTNMVRE LLIQFYKSTR
   661  FKPTRIIYYR GGVSEGQMKQ VAWPELIAIR KACISLEEDY RPGITYIVVQ KRHHTRLFCA
   721  DKTERVGKSG NVPAGTTVDS TITHPSEFDF YLCSHAGIQG TSRPSHYQVL WDDNCFTADE
   781  LQLLTYQLCH TYVRCTRSVS IPAPAYYARL VAFRARYHLV DKDHDSAEGS HVSGQSNGRD
   841  PQALAKAVQI HHDTQHTMYF A

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against AGO4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.24
Highest tissue expression: 19 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 19 nTPM
skin: 19 nTPM
retina: 17 nTPM
spleen: 13 nTPM
skeletal muscle: 12 nTPM
tongue: 11 nTPM

Single-cell type

neutrophils: 795 nCPM
neutrophil progenitors: 372 nCPM
monocyte progenitors: 208 nCPM
microglia: 191 nCPM
rod photoreceptor cells: 184 nCPM
monocytes: 158 nCPM

Immune cell

neutrophil: 7.5 nTPM
eosinophil: 3 nTPM
classical monocyte: 2.6 nTPM
myeloid DC: 2 nTPM
intermediate monocyte: 1.5 nTPM
MAIT T-cell: 1.4 nTPM

Brain region

choroid plexus: 17 nTPM
cerebellum: 16 nTPM
medulla oblongata: 14 nTPM
thalamus: 14 nTPM
white matter: 14 nTPM
midbrain: 14 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.22
gnomAD pLI: 1
gnomAD missense Z: 3.79
DepMap mean gene effect: -0.09
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of AGO4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads AGO4 as an antibody target. Whether an autoantibody or antibody against AGO4 could matter depends on whether native AGO4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

AGO4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label AGO4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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