Seroatlas · Human Serome Atlas

BTF3

Transcription factor BTF3

Also known as: BTF3_HUMAN, BTF3a, BTF3b, NACB

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P20290
Gene: BTF3
Ensembl: ENSG00000145741
Chromosome: 5
Canonical length: 206 aa
Protein class: Plasma proteins, Predicted intracellular proteins
Subcellular location: Cytosol

OverviewNCBI Gene

This gene encodes the basic transcription factor 3. This protein forms a stable complex with RNA polymerase IIB and is required for transcriptional initiation. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene has multiple pseudogenes. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

206 residues, UniProt reviewed canonical sequence.

>P20290|BTF3
     1  MRRTGAPAQA DSRGRGRARG GCPGGEATLS QPPPRGGTRG QEPQMKETIM NQEKLAKLQA
    61  QVRIGGKGTA RRKKKVVHRT ATADDKKLQF SLKKLGVNNI SGIEEVNMFT NQGTVIHFNN
   121  PKVQASLAAN TFTITGHAET KQLTEMLPSI LNQLGADSLT SLRRLAEALP KQSVDGKAPL
   181  ATGEDDDDEV PDLVENFDEA SKNEAN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against BTF3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.58
Highest tissue expression: 1,060 nTPM

Expression across tissuesHPA

Tissue

ovary: 1,060 nTPM
skeletal muscle: 850 nTPM
bone marrow: 812 nTPM
skin: 749 nTPM
breast: 714 nTPM
tonsil: 710 nTPM

Single-cell type

esophageal apical cells: 3,272 nCPM
esophageal suprabasal cells: 2,694 nCPM
decidual stromal cells: 2,548 nCPM
esophageal basal cells: 2,528 nCPM
extravillous trophoblasts: 2,368 nCPM
migrating cytotrophoblasts: 2,003 nCPM

Immune cell

total PBMC: 2,130 nTPM
T-reg: 1,148 nTPM
myeloid DC: 1,141 nTPM
naive CD4 T-cell: 988 nTPM
memory B-cell: 960 nTPM
memory CD4 T-cell: 908 nTPM

Brain region

white matter: 211 nTPM
hypothalamus: 200 nTPM
medulla oblongata: 191 nTPM
spinal cord: 189 nTPM
pons: 175 nTPM
cerebellum: 173 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.53
gnomAD pLI: 0.76
gnomAD missense Z: 1.53
DepMap mean gene effect: -0.83
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

in utero embryonic development
protein transport
negative regulation of protein localization to endoplasmic reticulum

Molecular functions

RNA binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of BTF3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads BTF3 as an antibody target. Whether an autoantibody or antibody against BTF3 could matter depends on whether native BTF3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

BTF3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label BTF3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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