DNAJA1
DnaJ homolog subfamily A member 1
Also known as: dj-2, DNJA1_HUMAN, hdj-2, HSJ2, HSPF4, NEDD7
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- P31689
- Gene
- DNAJA1
- Ensembl
- ENSG00000086061
- Chromosome
- 9
- Canonical length
- 397 aa
- Protein class
- Predicted intracellular proteins
- Subcellular location
- Microtubules,Cytosol
OverviewNCBI Gene
This gene encodes a member of the DnaJ family of proteins, which act as heat shock protein 70 cochaperones. Heat shock proteins facilitate protein folding, trafficking, prevention of aggregation, and proteolytic degradation. Members of this family are characterized by a highly conserved N-terminal J domain, a glycine/phenylalanine-rich region, four CxxCxGxG zinc finger repeats, and a C-terminal substrate-binding domain. The J domain mediates the interaction with heat shock protein 70 to recruit substrates and regulate ATP hydrolysis activity. In humans, this gene has been implicated in positive regulation of virus replication through co-option by the influenza A virus. Several pseudogenes of this gene are found on other chromosomes. [provided by RefSeq, Sep 2015]
Canonical amino-acid sequenceUniProt
397 residues, UniProt reviewed canonical sequence.
>P31689|DNAJA1
1 MVKETTYYDV LGVKPNATQE ELKKAYRKLA LKYHPDKNPN EGEKFKQISQ AYEVLSDAKK
61 RELYDKGGEQ AIKEGGAGGG FGSPMDIFDM FFGGGGRMQR ERRGKNVVHQ LSVTLEDLYN
121 GATRKLALQK NVICDKCEGR GGKKGAVECC PNCRGTGMQI RIHQIGPGMV QQIQSVCMEC
181 QGHGERISPK DRCKSCNGRK IVREKKILEV HIDKGMKDGQ KITFHGEGDQ EPGLEPGDII
241 IVLDQKDHAV FTRRGEDLFM CMDIQLVEAL CGFQKPISTL DNRTIVITSH PGQIVKHGDI
301 KCVLNEGMPI YRRPYEKGRL IIEFKVNFPE NGFLSPDKLS LLEKLLPERK EVEETDEMDQ
361 VELVDFDPNQ ERRRHYNGEA YEDDEHHPRG GVQCQTSLocalizationUniProt · AlphaFold · HPA
Whether an antibody against DNAJA1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.41
- Highest tissue expression
- 203 nTPM
Expression across tissuesHPA
Tissue
- liver: 203 nTPM
- bone marrow: 141 nTPM
- adrenal gland: 127 nTPM
- testis: 126 nTPM
- urinary bladder: 122 nTPM
- cerebral cortex: 122 nTPM
Single-cell type
- oocytes: 926 nCPM
- late primary spermatocytes: 916 nCPM
- pancreatic duct cells: 909 nCPM
- neutrophils: 781 nCPM
- schwann cells: 692 nCPM
- syncytiotrophoblasts: 670 nCPM
Immune cell
- non-classical monocyte: 270 nTPM
- plasmacytoid DC: 214 nTPM
- total PBMC: 210 nTPM
- intermediate monocyte: 202 nTPM
- neutrophil: 177 nTPM
- eosinophil: 161 nTPM
Brain region
- white matter: 158 nTPM
- hypothalamus: 142 nTPM
- cerebral cortex: 139 nTPM
- pons: 138 nTPM
- spinal cord: 124 nTPM
- hippocampal formation: 120 nTPM
DiseaseUniProt · ClinVar · IEDB · PubMed
Four sources answering four different questions about DNAJA1.
Disease | ImmuneIEDB
Conditions an epitope on DNAJA1 was assayed in.
- viral infectious disease T cell
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.54
- gnomAD pLI
- 0.09
- gnomAD missense Z
- 2.23
- DepMap mean gene effect
- -0.43
- DepMap dependency class
- selective
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- negative regulation of apoptotic process
- negative regulation of establishment of protein localization to mitochondrion
- negative regulation of JUN kinase activity
- negative regulation of nitrosative stress-induced intrinsic apoptotic signaling pathway
- negative regulation of protein ubiquitination
- positive regulation of apoptotic process
- protein folding
- protein localization to mitochondrion
- protein refolding
- regulation of protein transport
- response to heat
- response to unfolded protein
Molecular functions
- ATP binding
- ATPase activator activity
- C3HC4-type RING finger domain binding
- G protein-coupled receptor binding
- Hsp70 protein binding
- low-density lipoprotein particle receptor binding
- protein-folding chaperone binding
- Tat protein binding
- ubiquitin protein ligase binding
- unfolded protein binding
- zinc ion binding
Cellular components
Protein domainsUniProt · Pfam · InterPro
- Heat shock protein DnaJ, cysteine-rich domain
- DnaJ domain
- Chaperone DnaJ, C-terminal
- HSP40/DnaJ peptide-binding
- Chaperone DnaJ
- DnaJ domain, conserved site
- Heat shock protein DnaJ, cysteine-rich domain superfamily
- Chaperone J-domain superfamily
- DnaJ homolog subfamily A member 1/2-like
- DnaJ domain
- DnaJ central domain
- DnaJ C terminal domain
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of DNAJA1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads DNAJA1 as an antibody target. Whether an autoantibody or antibody against DNAJA1 could matter depends on whether native DNAJA1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
DNAJA1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label DNAJA1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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