Seroatlas · Human Serome Atlas

METTL3

N(6)-adenosine-methyltransferase catalytic subunit METTL3

Also known as: M6A, MT-A70, MTA70_HUMAN, Spo8

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q86U44
Gene
METTL3
Ensembl
ENSG00000165819
Chromosome
14
Canonical length
580 aa
Protein class
Enzymes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Nuclear bodies,Golgi apparatus,Cytosol

OverviewNCBI Gene

This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

580 residues, UniProt reviewed canonical sequence.

>Q86U44|METTL3
     1  MSDTWSSIQA HKKQLDSLRE RLQRRRKQDS GHLDLRNPEA ALSPTFRSDS PVPTAPTSGG
    61  PKPSTASAVP ELATDPELEK KLLHHLSDLA LTLPTDAVSI CLAISTPDAP ATQDGVESLL
   121  QKFAAQELIE VKRGLLQDDA HPTLVTYADH SKLSAMMGAV AEKKGPGEVA GTVTGQKRRA
   181  EQDSTTVAAF ASSLVSGLNS SASEPAKEPA KKSRKHAASD VDLEIESLLN QQSTKEQQSK
   241  KVSQEILELL NTTTAKEQSI VEKFRSRGRA QVQEFCDYGT KEECMKASDA DRPCRKLHFR
   301  RIINKHTDES LGDCSFLNTC FHMDTCKYVH YEIDACMDSE APGSKDHTPS QELALTQSVG
   361  GDSSADRLFP PQWICCDIRY LDVSILGKFA VVMADPPWDI HMELPYGTLT DDEMRRLNIP
   421  VLQDDGFLFL WVTGRAMELG RECLNLWGYE RVDEIIWVKT NQLQRIIRTG RTGHWLNHGK
   481  EHCLVGVKGN PQGFNQGLDC DVIVAEVRST SHKPDEIYGM IERLSPGTRK IELFGRPHNV
   541  QPNWITLGNQ LDGIHLLDPD VVARFKQRYP DGIISKPKNL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against METTL3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.43
Highest tissue expression
20 nTPM

Expression across tissuesHPA

Tissue

  • tonsil: 20 nTPM
  • testis: 20 nTPM
  • parathyroid gland: 18 nTPM
  • lymph node: 18 nTPM
  • fallopian tube: 18 nTPM
  • adrenal gland: 18 nTPM

Single-cell type

  • late primary spermatocytes: 177 nCPM
  • early spermatids: 177 nCPM
  • tuft cells: 58 nCPM
  • syncytiotrophoblasts: 54 nCPM
  • cytotrophoblasts: 47 nCPM
  • migrating cytotrophoblasts: 39 nCPM

Immune cell

  • NK-cell: 16 nTPM
  • T-reg: 16 nTPM
  • memory B-cell: 14 nTPM
  • plasmacytoid DC: 13 nTPM
  • MAIT T-cell: 13 nTPM
  • intermediate monocyte: 12 nTPM

Brain region

  • medulla oblongata: 9.7 nTPM
  • pons: 8.8 nTPM
  • thalamus: 8.5 nTPM
  • white matter: 8.4 nTPM
  • cerebral cortex: 8.1 nTPM
  • midbrain: 7.8 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.79
gnomAD pLI
0
gnomAD missense Z
3.05
DepMap mean gene effect
-0.57
DepMap dependency class
common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of METTL3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads METTL3 as an antibody target. Whether an autoantibody or antibody against METTL3 could matter depends on whether native METTL3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

METTL3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label METTL3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/METTL3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

© 2026 · Seroatlas, Discover Oregon LLC · Terms of use

Loading the interactive Seroatlas protein explorer...