Seroatlas · Human Serome Atlas

DDX50

ATP-dependent RNA helicase DDX50

Also known as: DDX50_HUMAN, GU2, GUB, MGC3199, RH-II/GuB

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9BQ39
Gene
DDX50
Ensembl
ENSG00000107625
Chromosome
10
Canonical length
737 aa
Protein class
Enzymes, Plasma proteins, Predicted intracellular proteins
Subcellular location
Nucleoli

OverviewNCBI Gene

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box enzyme that may be involved in ribosomal RNA synthesis or processing. This gene and DDX21, also called RH-II/GuA, have similar genomic structures and are in tandem orientation on chromosome 10, suggesting that the two genes arose by gene duplication in evolution. This gene has pseudogenes on chromosomes 2, 3 and 4. Alternative splicing of this gene generates multiple transcript variants, but the full length nature of all the other variants but one has not been defined. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

737 residues, UniProt reviewed canonical sequence.

>Q9BQ39|DDX50
     1  MPGKLLWGDI MELEAPLEES ESQKKERQKS DRRKSRHHYD SDEKSETREN GVTDDLDAPK
    61  AKKSKMKEKL NGDTEEGFNR LSDEFSKSHK SRRKDLPNGD IDEYEKKSKR VSSLDTSTHK
   121  SSDNKLEETL TREQKEGAFS NFPISEETIK LLKGRGVTYL FPIQVKTFGP VYEGKDLIAQ
   181  ARTGTGKTFS FAIPLIERLQ RNQETIKKSR SPKVLVLAPT RELANQVAKD FKDITRKLSV
   241  ACFYGGTSYQ SQINHIRNGI DILVGTPGRI KDHLQSGRLD LSKLRHVVLD EVDQMLDLGF
   301  AEQVEDIIHE SYKTDSEDNP QTLLFSATCP QWVYKVAKKY MKSRYEQVDL VGKMTQKAAT
   361  TVEHLAIQCH WSQRPAVIGD VLQVYSGSEG RAIIFCETKK NVTEMAMNPH IKQNAQCLHG
   421  DIAQSQREIT LKGFREGSFK VLVATNVAAR GLDIPEVDLV IQSSPPQDVE SYIHRSGRTG
   481  RAGRTGICIC FYQPRERGQL RYVEQKAGIT FKRVGVPSTM DLVKSKSMDA IRSLASVSYA
   541  AVDFFRPSAQ RLIEEKGAVD ALAAALAHIS GASSFEPRSL ITSDKGFVTM TLESLEEIQD
   601  VSCAWKELNR KLSSNAVSQI TRMCLLKGNM GVCFDVPTTE SERLQAEWHD SDWILSVPAK
   661  LPEIEEYYDG NTSSNSRQRS GWSSGRSGRS GRSGGRSGGR SGRQSRQGSR SGSRQDGRRR
   721  SGNRNRSRSG GHKRSFD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DDX50 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.38
Highest tissue expression
30 nTPM

Expression across tissuesHPA

Tissue

  • ovary: 30 nTPM
  • blood vessel: 30 nTPM
  • thymus: 29 nTPM
  • lymph node: 26 nTPM
  • tonsil: 26 nTPM
  • endometrium: 26 nTPM

Single-cell type

  • rod photoreceptor cells: 145 nCPM
  • somatotrophs: 142 nCPM
  • sertoli cells: 139 nCPM
  • lactotrophs: 138 nCPM
  • early primary spermatocytes: 131 nCPM
  • cone photoreceptor cells: 122 nCPM

Immune cell

  • naive CD4 T-cell: 24 nTPM
  • MAIT T-cell: 23 nTPM
  • memory CD4 T-cell: 23 nTPM
  • naive CD8 T-cell: 22 nTPM
  • T-reg: 22 nTPM
  • NK-cell: 21 nTPM

Brain region

  • cerebellum: 19 nTPM
  • choroid plexus: 19 nTPM
  • white matter: 18 nTPM
  • hypothalamus: 17 nTPM
  • cerebral cortex: 15 nTPM
  • thalamus: 15 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.61
gnomAD pLI
0
gnomAD missense Z
2.07
DepMap mean gene effect
-0.15
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of DDX50 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DDX50 as an antibody target. Whether an autoantibody or antibody against DDX50 could matter depends on whether native DDX50 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DDX50 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DDX50 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/DDX50. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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