Seroatlas · Human Serome Atlas

COIL

Coilin

Also known as: CLN80, COIL_HUMAN, p80-coilin

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P38432
Gene: COIL
Ensembl: ENSG00000121058
Chromosome: 17
Canonical length: 576 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Nucleoli fibrillar center,Nuclear bodies

OverviewNCBI Gene

The protein encoded by this gene is an integral component of Cajal bodies (also called coiled bodies). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome 4 and chromosome 14. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

576 residues, UniProt reviewed canonical sequence.

>P38432|COIL
     1  MAASETVRLR LQFDYPPPAT PHCTAFWLLV DLNRCRVVTD LISLIRQRFG FSSGAFLGLY
    61  LEGGLLPPAE SARLVRDNDC LRVKLEERGV AENSVVISNG DINLSLRKAK KRAFQLEEGE
   121  ETEPDCKYSK KHWKSRENNN NNEKVLDLEP KAVTDQTVSK KNKRKNKATC GTVGDDNEEA
   181  KRKSPKKKEK CEYKKKAKNP KSPKVQAVKD WANQRCSSPK GSARNSLVKA KRKGSVSVCS
   241  KESPSSSSES ESCDESISDG PSKVTLEARN SSEKLPTELS KEEPSTKNTT ADKLAIKLGF
   301  SLTPSKGKTS GTTSSSSDSS AESDDQCLMS SSTPECAAGF LKTVGLFAGR GRPGPGLSSQ
   361  TAGAAGWRRS GSNGGGQAPG ASPSVSLPAS LGRGWGREEN LFSWKGAKGR GMRGRGRGRG
   421  HPVSCVVNRS TDNQRQQQLN DVVKNSSTII QNPVETPKKD YSLLPLLAAA PQVGEKIAFK
   481  LLELTSSYSP DVSDYKEGRI LSHNPETQQV DIEILSSLPA LREPGKFDLV YHNENGAEVV
   541  EYAVTQESKI TVFWKELIDP RLIIESPSNT SSTEPA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against COIL can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.61
Highest tissue expression: 92 nTPM

Expression across tissuesHPA

Tissue

testis: 92 nTPM
bone marrow: 19 nTPM
tonsil: 13 nTPM
thymus: 12 nTPM
placenta: 12 nTPM
liver: 12 nTPM

Single-cell type

late spermatids: 905 nCPM
late primary spermatocytes: 267 nCPM
early spermatids: 224 nCPM
oocytes: 124 nCPM
early primary spermatocytes: 72 nCPM
cytotrophoblasts: 53 nCPM

Immune cell

memory B-cell: 28 nTPM
naive B-cell: 25 nTPM
basophil: 22 nTPM
naive CD4 T-cell: 20 nTPM
naive CD8 T-cell: 19 nTPM
T-reg: 19 nTPM

Brain region

cerebellum: 16 nTPM
white matter: 12 nTPM
cerebral cortex: 11 nTPM
pons: 11 nTPM
choroid plexus: 11 nTPM
hypothalamus: 9.9 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about COIL.

Disease | ImmuneIEDB

Conditions an epitope on COIL was assayed in.

systemic scleroderma B cell

ReferencesPubMed · IEDB

Publications for COIL from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Reference: AutoantibodyPubMed

5 publications

Human autoantibody to a novel protein of the nuclear coiled body: immunological characterization and cDNA cloning of p80-coilin.
1991 · J Exp Med · RCR 6.4 · 330 citations
Anti-p80 coilin autoantibodies react with a conserved epitope and are associated with anti-DFS70/LEDGF autoantibodies.
2006 · J Autoimmun · RCR 0.6 · 22 citations
The behavior of the coiled body in cells infected with adenovirus in vitro.
1996 · Mol Biol Rep · RCR 0.4 · 20 citations
Clinical features and IgG subclass distribution of anti-p80 coilin antibodies.
1999 · J Autoimmun · RCR 0.3 · 10 citations
Distribution of anti-p80-coilin autoantibody in collagen diseases and various skin diseases.
1997 · Br J Dermatol · RCR 0.2 · 7 citations

Reference: B cellIEDB

1 publication

A population of autoantibodies against a centromere-associated protein A major epitope motif cross-reacts with related cryptic epitopes on other nuclear autoantigens and on the Epstein-Barr nuclear antigen 1.
2001 · J Mol Med (Berl) · RCR 0.6 · 28 citations

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. IEDB — curated epitope assays from the Immune Epitope Database (Vita et al., Nucleic Acids Research 2019). Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.59
gnomAD pLI: 0.01
gnomAD missense Z: 0.17
DepMap mean gene effect: -0.4
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 10% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

spliceosomal snRNP assembly

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Coilin
Coilin, N-terminal domain
Coilin, tudor domain
Coilin N-terminus
Coilin, C-terminal Tudor domain

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of COIL in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads COIL as an antibody target. Whether an autoantibody or antibody against COIL could matter depends on whether native COIL is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

COIL is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label COIL as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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