Seroatlas · Human Serome Atlas

MFAP1

Microfibrillar-associated protein 1

Also known as: AMP, MFAP1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P55081
Gene: MFAP1
Ensembl: ENSG00000140259
Chromosome: 15
Canonical length: 439 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Centrosome

OverviewNCBI Gene

Enables RNA binding activity. Involved in mRNA splicing, via spliceosome. Located in centrosome; microfibril; and nucleoplasm. Part of U2-type precatalytic spliceosome. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

439 residues, UniProt reviewed canonical sequence.

>P55081|MFAP1
     1  MSVPSALMKQ PPIQSTAGAV PVRNEKGEIS MEKVKVKRYV SGKRPDYAPM ESSDEEDEEF
    61  QFIKKAKEQE AEPEEQEEDS SSDPRLRRLQ NRISEDVEER LARHRKIVEP EVVGESDSEV
   121  EGDAWRMERE DSSEEEEEEI DDEEIERRRG MMRQRAQERK NEEMEVMEVE DEGRSGEESE
   181  SESEYEEYTD SEDEMEPRLK PVFIRKKDRV TVQEREAEAL KQKELEQEAK RMAEERRKYT
   241  LKIVEEETKK ELEENKRSLA ALDALNTDDE NDEEEYEAWK VRELKRIKRD REDREALEKE
   301  KAEIERMRNL TEEERRAELR ANGKVITNKA VKGKYKFLQK YYHRGAFFMD EDEEVYKRDF
   361  SAPTLEDHFN KTILPKVMQV KNFGRSGRTK YTHLVDQDTT SFDSAWGQES AQNTKFFKQK
   421  AAGVRDVFER PSAKKRKTT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MFAP1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.57
Highest tissue expression: 31 nTPM

Expression across tissuesHPA

Tissue

parathyroid gland: 31 nTPM
tonsil: 30 nTPM
choroid plexus: 28 nTPM
skeletal muscle: 25 nTPM
lymph node: 25 nTPM
adrenal gland: 24 nTPM

Single-cell type

syncytiotrophoblasts: 184 nCPM
cytotrophoblasts: 121 nCPM
esophageal apical cells: 121 nCPM
differentiating spermatogonia: 107 nCPM
migrating cytotrophoblasts: 98 nCPM
erythrocyte progenitors: 86 nCPM

Immune cell

eosinophil: 57 nTPM
NK-cell: 51 nTPM
T-reg: 44 nTPM
basophil: 43 nTPM
naive CD4 T-cell: 36 nTPM
gdT-cell: 35 nTPM

Brain region

cerebellum: 24 nTPM
choroid plexus: 21 nTPM
hypothalamus: 21 nTPM
midbrain: 20 nTPM
cerebral cortex: 20 nTPM
white matter: 19 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.37
gnomAD pLI: 0.8
gnomAD missense Z: 2.74
DepMap mean gene effect: -1.79
DepMap dependency class: pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

via spliceosome
mRNA splicing

Molecular functions

RNA binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

Micro-fibrillar-associated protein 1, C-terminal
Microfibrillar-associated protein 1
Microfibril-associated/Pre-mRNA processing

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MFAP1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MFAP1 as an antibody target. Whether an autoantibody or antibody against MFAP1 could matter depends on whether native MFAP1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MFAP1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MFAP1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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