Seroatlas · Human Serome Atlas

CHEK1

Serine/threonine-protein kinase Chk1

Also known as: CHK1, CHK1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O14757
Gene
CHEK1
Ensembl
ENSG00000149554
Chromosome
11
Canonical length
476 aa
Protein class
Cancer-related genes, Enzymes, Plasma proteins, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Vesicles

OverviewNCBI Gene

The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011]

Canonical amino-acid sequenceUniProt

476 residues, UniProt reviewed canonical sequence.

>O14757|CHEK1
     1  MAVPFVEDWD LVQTLGEGAY GEVQLAVNRV TEEAVAVKIV DMKRAVDCPE NIKKEICINK
    61  MLNHENVVKF YGHRREGNIQ YLFLEYCSGG ELFDRIEPDI GMPEPDAQRF FHQLMAGVVY
   121  LHGIGITHRD IKPENLLLDE RDNLKISDFG LATVFRYNNR ERLLNKMCGT LPYVAPELLK
   181  RREFHAEPVD VWSCGIVLTA MLAGELPWDQ PSDSCQEYSD WKEKKTYLNP WKKIDSAPLA
   241  LLHKILVENP SARITIPDIK KDRWYNKPLK KGAKRPRVTS GGVSESPSGF SKHIQSNLDF
   301  SPVNSASSEE NVKYSSSQPE PRTGLSLWDT SPSYIDKLVQ GISFSQPTCP DHMLLNSQLL
   361  GTPGSSQNPW QRLVKRMTRF FTKLDADKSY QCLKETCEKL GYQWKKSCMN QVTISTTDRR
   421  NNKLIFKVNL LEMDDKILVD FRLSKGDGLE FKRHFLKIKG KLIDIVSSQK IWLPAT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CHEK1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.3
Highest tissue expression
29 nTPM

Expression across tissuesHPA

Tissue

  • seminal vesicle: 29 nTPM
  • bone marrow: 25 nTPM
  • thymus: 20 nTPM
  • lymph node: 14 nTPM
  • tonsil: 13 nTPM
  • parathyroid gland: 13 nTPM

Single-cell type

  • oocytes: 143 nCPM
  • erythrocyte progenitors: 100 nCPM
  • epididymal basal cells: 92 nCPM
  • early primary spermatocytes: 87 nCPM
  • megakaryocyte progenitors: 87 nCPM
  • monocyte progenitors: 74 nCPM

Immune cell

  • T-reg: 6.5 nTPM
  • naive CD4 T-cell: 2.3 nTPM
  • memory CD4 T-cell: 2.2 nTPM
  • basophil: 1.6 nTPM
  • memory B-cell: 1.4 nTPM
  • naive CD8 T-cell: 1.4 nTPM

Brain region

  • thalamus: 3.7 nTPM
  • cerebral cortex: 3.3 nTPM
  • basal ganglia: 3.1 nTPM
  • hypothalamus: 3 nTPM
  • midbrain: 2.8 nTPM
  • white matter: 2.8 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about CHEK1.

Disease | AllUniProt

Conditions CHEK1 is implicated in, by any mechanism.

  • Oocyte/zygote/embryo maturation arrest 21 (OZEMA21) MIM:620610

Disease | GeneticClinVar

5 pathogenic / likely-pathogenic of 111 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

  • Oocyte/zygote/embryo maturation arrest 21
  • Male infertility due to gonadal dysgenesis or sperm disorder

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.93
gnomAD pLI
0
gnomAD missense Z
1.78
DepMap mean gene effect
-1.89
DepMap dependency class
pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CHEK1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CHEK1 as an antibody target. Whether an autoantibody or antibody against CHEK1 could matter depends on whether native CHEK1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CHEK1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CHEK1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/CHEK1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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