Seroatlas · Human Serome Atlas

CDC25B

M-phase inducer phosphatase 2

Also known as: MPIP2_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P30305
Gene: CDC25B
Ensembl: ENSG00000101224
Chromosome: 20
Canonical length: 580 aa
Protein class: Cancer-related genes, Enzymes, Plasma proteins, Predicted intracellular proteins
Subcellular location: Vesicles,Mitotic spindle

OverviewNCBI Gene

CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

580 residues, UniProt reviewed canonical sequence.

>P30305|CDC25B
     1  MEVPQPEPAP GSALSPAGVC GGAQRPGHLP GLLLGSHGLL GSPVRAAASS PVTTLTQTMH
    61  DLAGLGSETP KSQVGTLLFR SRSRLTHLSL SRRASESSLS SESSESSDAG LCMDSPSPMD
   121  PHMAEQTFEQ AIQAASRIIR NEQFAIRRFQ SMPVRLLGHS PVLRNITNSQ APDGRRKSEA
   181  GSGAASSSGE DKENDGFVFK MPWKPTHPSS THALAEWASR REAFAQRPSS APDLMCLSPD
   241  RKMEVEELSP LALGRFSLTP AEGDTEEDDG FVDILESDLK DDDAVPPGME SLISAPLVKT
   301  LEKEEEKDLV MYSKCQRLFR SPSMPCSVIR PILKRLERPQ DRDTPVQNKR RRSVTPPEEQ
   361  QEAEEPKARV LRSKSLCHDE IENLLDSDHR ELIGDYSKAF LLQTVDGKHQ DLKYISPETM
   421  VALLTGKFSN IVDKFVIVDC RYPYEYEGGH IKTAVNLPLE RDAESFLLKS PIAPCSLDKR
   481  VILIFHCEFS SERGPRMCRF IRERDRAVND YPSLYYPEMY ILKGGYKEFF PQHPNFCEPQ
   541  DYRPMNHEAF KDELKTFRLK TRSWAGERSR RELCSRLQDQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CDC25B can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.56
Highest tissue expression: 87 nTPM

Expression across tissuesHPA

Tissue

cerebellum: 87 nTPM
lung: 76 nTPM
spleen: 55 nTPM
pituitary gland: 45 nTPM
lymph node: 45 nTPM
thymus: 42 nTPM

Single-cell type

retinal ganglion cells: 66 nCPM
brain inhibitory neurons: 63 nCPM
corticotrophs: 62 nCPM
brain excitatory neurons: 61 nCPM
other brain neurons: 59 nCPM
hofbauer cells: 56 nCPM

Immune cell

T-reg: 28 nTPM
memory CD8 T-cell: 23 nTPM
gdT-cell: 23 nTPM
memory CD4 T-cell: 23 nTPM
naive CD8 T-cell: 22 nTPM
MAIT T-cell: 21 nTPM

Brain region

cerebellum: 50 nTPM
white matter: 43 nTPM
cerebral cortex: 37 nTPM
hypothalamus: 36 nTPM
basal ganglia: 36 nTPM
medulla oblongata: 34 nTPM

ReferencesPubMed · IEDB

Publications for CDC25B from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Reference: AutoantibodyPubMed

2 publications

Anti-CDC25B autoantibody predicts poor prognosis in patients with advanced esophageal squamous cell carcinoma.
2010 · J Transl Med · RCR 1 · 41 citations
Proteomics-based identification of autoantibody against CDC25B as a novel serum marker in esophageal squamous cell carcinoma.
2008 · Biochem Biophys Res Commun · RCR 0.7 · 26 citations

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.43
gnomAD pLI: 0.36
gnomAD missense Z: 1.47
DepMap mean gene effect: -0.33
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CDC25B in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CDC25B as an antibody target. Whether an autoantibody or antibody against CDC25B could matter depends on whether native CDC25B is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CDC25B is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CDC25B as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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