Seroatlas · Human Serome Atlas

CENPO

Centromere protein O

Also known as: CENP-O, CENPO_HUMAN, MGC11266

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9BU64
Gene
CENPO
Ensembl
ENSG00000138092
Chromosome
2
Canonical length
300 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm,Nuclear bodies

OverviewNCBI Gene

This gene encodes a component of the interphase centromere complex. The encoded protein is localized to the centromere throughout the cell cycle and is required for bipolar spindle assembly, chromosome segregation and checkpoint signaling during mitosis. Alternatively spliced transcript variants encoding multiple protein isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]

Canonical amino-acid sequenceUniProt

300 residues, UniProt reviewed canonical sequence.

>Q9BU64|CENPO
     1  MEQANPLRPD GESKGGVLAH LERLETQVSR SRKQSEELQS VQAQEGALGT KIHKLRRLRD
    61  ELRAVVRHRR ASVKACIANV EPNQTVEINE QEALEEKLEN VKAILQAYHF TGLSGKLTSR
   121  GVCVCISTAF EGNLLDSYFV DLVIQKPLRI HHHSVPVFIP LEEIAAKYLQ TNIQHFLFSL
   181  CEYLNAYSGR KYQADRLQSD FAALLTGPLQ RNPLCNLLSF TYKLDPGGQS FPFCARLLYK
   241  DLTATLPTDV TVTCQGVEVL STSWEEQRAS HETLFCTKPL HQVFASFTRK GEKLDMSLVS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CENPO can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.39
Highest tissue expression
11 nTPM

Expression across tissuesHPA

Tissue

  • thymus: 11 nTPM
  • bone marrow: 8.7 nTPM
  • testis: 8.1 nTPM
  • tonsil: 5.5 nTPM
  • adipose tissue: 5 nTPM
  • cerebral cortex: 4.7 nTPM

Single-cell type

  • epicardial cells: 39 nCPM
  • erythrocyte progenitors: 38 nCPM
  • pericytes: 34 nCPM
  • monocyte progenitors: 31 nCPM
  • differentiating spermatogonia: 30 nCPM
  • extravillous trophoblasts: 29 nCPM

Immune cell

  • plasmacytoid DC: 1.1 nTPM
  • gdT-cell: 1 nTPM
  • naive CD8 T-cell: 0.7 nTPM
  • NK-cell: 0.7 nTPM
  • T-reg: 0.7 nTPM
  • memory CD8 T-cell: 0.5 nTPM

Brain region

  • hypothalamus: 7.8 nTPM
  • medulla oblongata: 7.7 nTPM
  • hippocampal formation: 7.6 nTPM
  • pons: 7.3 nTPM
  • amygdala: 7.1 nTPM
  • cerebral cortex: 7.1 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.3
gnomAD pLI
0
gnomAD missense Z
0.4
DepMap mean gene effect
-0.16
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • Centromere protein O
  • Cenp-O kinetochore centromere component

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CENPO in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CENPO as an antibody target. Whether an autoantibody or antibody against CENPO could matter depends on whether native CENPO is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CENPO is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CENPO as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/CENPO. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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