Seroatlas · Human Serome Atlas

E2F4

Transcription factor E2F4

Also known as: E2F-4, E2F4_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q16254
Gene
E2F4
Ensembl
ENSG00000205250
Chromosome
16
Canonical length
413 aa
Protein class
Predicted intracellular proteins, Transcription factors
Subcellular location
Nucleoplasm

OverviewNCBI Gene

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

413 residues, UniProt reviewed canonical sequence.

>Q16254|E2F4
     1  MAEAGPQAPP PPGTPSRHEK SLGLLTTKFV SLLQEAKDGV LDLKLAADTL AVRQKRRIYD
    61  ITNVLEGIGL IEKKSKNSIQ WKGVGPGCNT REIADKLIEL KAEIEELQQR EQELDQHKVW
   121  VQQSIRNVTE DVQNSCLAYV THEDICRCFA GDTLLAIRAP SGTSLEVPIP EGLNGQKKYQ
   181  IHLKSVSGPI EVLLVNKEAW SSPPVAVPVP PPEDLLQSPS AVSTPPPLPK PALAQSQEAS
   241  RPNSPQLTPT AVPGSAEVQG MAGPAAEITV SGGPGTDSKD SGELSSLPLG PTTLDTRPLQ
   301  SSALLDSSSS SSSSSSSSSN SNSSSSSGPN PSTSFEPIKA DPTGVLELPK ELSEIFDPTR
   361  ECMSSELLEE LMSSEVFAPL LRLSPPPGDH DYIYNLDESE GVCDLFDVPV LNL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against E2F4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.57
Highest tissue expression
93 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 93 nTPM
  • spleen: 52 nTPM
  • skeletal muscle: 48 nTPM
  • esophagus: 46 nTPM
  • lymph node: 45 nTPM
  • small intestine: 42 nTPM

Single-cell type

  • late spermatids: 143 nCPM
  • cytotrophoblasts: 136 nCPM
  • migrating cytotrophoblasts: 129 nCPM
  • erythrocyte progenitors: 128 nCPM
  • extravillous trophoblasts: 89 nCPM
  • syncytiotrophoblasts: 78 nCPM

Immune cell

  • myeloid DC: 15 nTPM
  • classical monocyte: 12 nTPM
  • eosinophil: 11 nTPM
  • intermediate monocyte: 11 nTPM
  • memory CD8 T-cell: 9.9 nTPM
  • NK-cell: 8.7 nTPM

Brain region

  • cerebral cortex: 23 nTPM
  • hypothalamus: 23 nTPM
  • medulla oblongata: 22 nTPM
  • pons: 21 nTPM
  • spinal cord: 20 nTPM
  • thalamus: 20 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.49
gnomAD pLI
0.45
gnomAD missense Z
1.09
DepMap mean gene effect
-0.12
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of E2F4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads E2F4 as an antibody target. Whether an autoantibody or antibody against E2F4 could matter depends on whether native E2F4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

E2F4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label E2F4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/E2F4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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