Seroatlas · Human Serome Atlas

CASP3

Caspase-3

Also known as: apopain, CASP3_HUMAN, CPP32, CPP32B, Yama

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P42574
Gene
CASP3
Ensembl
ENSG00000164305
Chromosome
4
Canonical length
277 aa
Protein class
Cancer-related genes, Enzymes, FDA approved drug targets, Plasma proteins, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Mitochondria

OverviewNCBI Gene

The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017]

Canonical amino-acid sequenceUniProt

277 residues, UniProt reviewed canonical sequence.

>P42574|CASP3
     1  MENTENSVDS KSIKNLEPKI IHGSESMDSG ISLDNSYKMD YPEMGLCIII NNKNFHKSTG
    61  MTSRSGTDVD AANLRETFRN LKYEVRNKND LTREEIVELM RDVSKEDHSK RSSFVCVLLS
   121  HGEEGIIFGT NGPVDLKKIT NFFRGDRCRS LTGKPKLFII QACRGTELDC GIETDSGVDD
   181  DMACHKIPVE ADFLYAYSTA PGYYSWRNSK DGSWFIQSLC AMLKQYADKL EFMHILTRVN
   241  RKVATEFESF SFDATFHAKK QIPCIVSMLT KELYFYH

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CASP3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.32
Highest tissue expression
48 nTPM

Expression across tissuesHPA

Tissue

  • duodenum: 48 nTPM
  • tonsil: 37 nTPM
  • small intestine: 36 nTPM
  • lymph node: 36 nTPM
  • thymus: 34 nTPM
  • colon: 30 nTPM

Single-cell type

  • platelets: 225 nCPM
  • innate lymphoid cells: 165 nCPM
  • megakaryocyte-erythroid progenitors: 164 nCPM
  • plasma cells: 151 nCPM
  • breast lactating cells: 130 nCPM
  • ocular epithelial cells: 105 nCPM

Immune cell

  • basophil: 491 nTPM
  • eosinophil: 333 nTPM
  • non-classical monocyte: 60 nTPM
  • T-reg: 52 nTPM
  • NK-cell: 52 nTPM
  • memory CD8 T-cell: 38 nTPM

Brain region

  • cerebellum: 17 nTPM
  • white matter: 9.8 nTPM
  • hypothalamus: 9.1 nTPM
  • midbrain: 8.1 nTPM
  • spinal cord: 8.1 nTPM
  • thalamus: 8.1 nTPM

ReferencesPubMed · IEDB

Publications for CASP3 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Reference: AutoantibodyPubMed

2 publications

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.69
gnomAD pLI
0.1
gnomAD missense Z
1.66
DepMap mean gene effect
0.17
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CASP3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CASP3 as an antibody target. Whether an autoantibody or antibody against CASP3 could matter depends on whether native CASP3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CASP3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CASP3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/CASP3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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