HTH CenpB-type DNA-binding domain
IPR006600
Definition
The CENPB-type HTH domain is a DNA-binding, helix-turn-helix (HTH) domain of about 70-75 amino acids, present in eukaryotic centromere proteins and transposases. The domain is named after the mammalian major centromere autoantigen B or centromere protein B (CENP-B), which is a fundamental centromere component of chromosomes. The N terminus of CENP-B contains two DNA-binding HTH domains, which bind to adjacent major grooves of DNA. The N terminus of CENP-B is formed by a psq-type HTH domain and C-terminal to this domain lies the CENPB-type HTH domain. These two HTH domains together bind specifically to a 17-base-pair sequence, the CENP-B box, which occurs in alpha-satellite DNA in human centromeres PMID:16183641. The structure of the CENPB-type HTH domain is composed of three α-helices. The second and third helices connected via a turn comprise the helix-turn-helix motif. Helix 3 is termed the recognition helix as it binds the DNA major groove, like in other HTHs. In CENP-B this domain recognises site 3 of the CENP-B box, while the preceding psq-type HTH binds site 1 of the CENP-B box, and a connecting linker loop binds in the minor groove of DNA and recognises site 2 PMID:11726497. Some proteins known to contain a CENPB-type HTH domain: Mammalian centromere protein B (CENP-B), associated with the centromere and specifically binding DNA to the CENP-B box. Mammalian jerky protein, involved in epileptic seizures in mice PMID:15487591. Mammalian Pogo transposases PMID:9862965 and tigger transposable elements PMID:9097724. Fission yeast ARS-binding protein 1 (abp1) PMID:17112379 and CENP-B homologue proteins (CBHP-1 and 2), which are centromere proteins [[cite:PMID:9237993], [cite:PMID:11238404]]. Candida albicans protein PDC2 (Pyruvate DeCarboxylase 2) [[cite:PMID:8264540], [cite:PMID:19013460]]. Fungal transposases.
13 human proteins with this domain
Each is a reviewed human protein in the Seroatlas serome and a potential autoantibody target; this domain groups them into one antibody-relevant category. Every entry links to its own record.
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