CENPB

Protein identityUniProt · HPA

UniProt accession

P07199

Gene

CENPB

Ensembl

ENSG00000125817

Chromosome

20

Canonical length

599 aa

Protein class

Predicted intracellular proteins

Subcellular location

Nucleoplasm,Nuclear bodies

Quaternary structure

Homodimer

OverviewNCBI Gene

This gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

599 residues, UniProt reviewed canonical sequence.

>P07199|CENPB
     1  MGPKRRQLTF REKSRIIQEV EENPDLRKGE IARRFNIPPS TLSTILKNKR AILASERKYG
    61  VASTCRKTNK LSPYDKLEGL LIAWFQQIRA AGLPVKGIIL KEKALRIAEE LGMDDFTASN
   121  GWLDRFRRRH GVVSCSGVAR ARARNAAPRT PAAPASPAAV PSEGSGGSTT GWRAREEQPP
   181  SVAEGYASQD VFSATETSLW YDFLPDQAAG LCGGDGRPRQ ATQRLSVLLC ANADGSEKLP
   241  PLVAGKSAKP RAGQAGLPCD YTANSKGGVT TQALAKYLKA LDTRMAAESR RVLLLAGRLA
   301  AQSLDTSGLR HVQLAFFPPG TVHPLERGVV QQVKGHYRQA MLLKAMAALE GQDPSGLQLG
   361  LTEALHFVAA AWQAVEPSDI AACFREAGFG GGPNATITTS LKSEGEEEEE EEEEEEEEEG
   421  EGEEEEEEGE EEEEEGGEGE ELGEEEEVEE EGDVDSDEEE EEDEESSSEG LEAEDWAQGV
   481  VEAGGSFGAY GAQEEAQCPT LHFLEGGEDS DSDSEEEDDE EEDDEDEDDD DDEEDGDEVP
   541  VPSFGEAMAY FAMVKRYLTS FPIDDRVQSH ILHLEHDLVH VTRKNHARQA GVRGLGHQS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CENPB can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.44

Highest tissue expression

170 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about CENPB.

Disease | ImmuneIEDB

Conditions an epitope on CENPB was assayed in.

• systemic scleroderma B cell

Disease | AutoantibodyPubMed

Conditions in which antibodies against CENPB are reported. Each links to that disease's full target list.

• Scleroderma, Systemic 23
• Liver Cirrhosis, Biliary 7
• Sjogren's Syndrome 7
• Arthritis, Rheumatoid 4
• Lupus Erythematosus, Systemic 4
• Breast Neoplasms 3

Showing 6 of 7 — disease pages carrying at least 10 antigens.

ReferencesPubMed · IEDB

Publications for CENPB from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. IEDB — curated epitope assays from the Immune Epitope Database (Vita et al., Nucleic Acids Research 2019). Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.18

gnomAD pLI

1

gnomAD missense Z

2.73

DepMap mean gene effect

-0.05

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Molecular functions

• centromeric DNA binding
• chromatin binding
• DNA binding
• satellite DNA binding
• sequence-specific DNA binding

Cellular components

• chromosome
• chromosome, centromeric region
• condensed chromosome, centromeric region
• nuclear body
• nucleoplasm
• nucleus
• pericentric heterochromatin

Protein domainsUniProt · Pfam · InterPro

• DDE superfamily endonuclease domain
• HTH CenpB-type DNA-binding domain
• DNA binding HTH domain, Psq-type
• Homedomain-like superfamily
• Centromere and Transposable Element-Derived Protein
• DDE superfamily endonuclease
• Tc5 transposase DNA-binding domain
• CENP-B N-terminal DNA-binding domain
• Centromere protein CENP-B, C-terminal domain
• CENP-B, dimerisation domain superfamily
• Centromere protein B dimerisation domain

KeywordsUniProt

• ADP-ribosylation
• Centromere
• Chromosome
• DNA-binding
• Isopeptide bond
• Methylation
• Nucleus
• Phosphoprotein
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of CENPB in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• CENPT
• CSNK2B