Seroatlas · Human Serome Atlas

TRIM25

E3 ubiquitin/ISG15 ligase TRIM25

Also known as: EFP, RNF147, TRI25_HUMAN, ZNF147

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q14258
Gene
TRIM25
Ensembl
ENSG00000121060
Chromosome
17
Canonical length
630 aa
Protein class
Cancer-related genes, Enzymes, Plasma proteins, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Nuclear bodies,Cytosol
Quaternary structure
Homodimer

OverviewNCBI Gene

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein is an RNA binding protein, functions as a ubiquitin E3 ligase and is involved in multiple cellular processes, including regulation of antiviral innate immunity. [provided by RefSeq, Sep 2021]

Canonical amino-acid sequenceUniProt

630 residues, UniProt reviewed canonical sequence.

>Q14258|TRIM25
     1  MAELCPLAEE LSCSICLEPF KEPVTTPCGH NFCGSCLNET WAVQGSPYLC PQCRAVYQAR
    61  PQLHKNTVLC NVVEQFLQAD LAREPPADVW TPPARASAPS PNAQVACDHC LKEAAVKTCL
   121  VCMASFCQEH LQPHFDSPAF QDHPLQPPVR DLLRRKCSQH NRLREFFCPE HSECICHICL
   181  VEHKTCSPAS LSQASADLEA TLRHKLTVMY SQINGASRAL DDVRNRQQDV RMTANRKVEQ
   241  LQQEYTEMKA LLDASETTST RKIKEEEKRV NSKFDTIYQI LLKKKSEIQT LKEEIEQSLT
   301  KRDEFEFLEK ASKLRGISTK PVYIPEVELN HKLIKGIHQS TIDLKNELKQ CIGRLQEPTP
   361  SSGDPGEHDP ASTHKSTRPV KKVSKEEKKS KKPPPVPALP SKLPTFGAPE QLVDLKQAGL
   421  EAAAKATSSH PNSTSLKAKV LETFLAKSRP ELLEYYIKVI LDYNTAHNKV ALSECYTVAS
   481  VAEMPQNYRP HPQRFTYCSQ VLGLHCYKKG IHYWEVELQK NNFCGVGICY GSMNRQGPES
   541  RLGRNSASWC VEWFNTKISA WHNNVEKTLP STKATRVGVL LNCDHGFVIF FAVADKVHLM
   601  YKFRVDFTEA LYPAFWVFSA GATLSICSPK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TRIM25 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.4
Highest tissue expression
46 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 46 nTPM
  • spleen: 40 nTPM
  • liver: 28 nTPM
  • skin: 28 nTPM
  • lung: 24 nTPM
  • esophagus: 19 nTPM

Single-cell type

  • neutrophils: 452 nCPM
  • endometrial luminal cells: 260 nCPM
  • endometrial glandular cells: 182 nCPM
  • monocytes: 135 nCPM
  • epicardial cells: 89 nCPM
  • retinal bipolar cells: 85 nCPM

Immune cell

  • neutrophil: 8.7 nTPM
  • myeloid DC: 2.9 nTPM
  • intermediate monocyte: 2.4 nTPM
  • basophil: 2.3 nTPM
  • classical monocyte: 2.3 nTPM
  • memory B-cell: 2.1 nTPM

Brain region

  • cerebral cortex: 27 nTPM
  • hypothalamus: 15 nTPM
  • spinal cord: 13 nTPM
  • thalamus: 13 nTPM
  • choroid plexus: 12 nTPM
  • medulla oblongata: 12 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.59
gnomAD pLI
0
gnomAD missense Z
0.86
DepMap mean gene effect
-0.09
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of TRIM25 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TRIM25 as an antibody target. Whether an autoantibody or antibody against TRIM25 could matter depends on whether native TRIM25 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TRIM25 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TRIM25 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/TRIM25. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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