TAF9

Protein identityUniProt · HPA

UniProt accession

Q16594

Gene

TAF9

Ensembl

ENSG00000273841

Chromosome

5

Canonical length

264 aa

Protein class

Metabolic proteins, Plasma proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm

OverviewNCBI Gene

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds to the basal transcription factor GTF2B as well as to several transcriptional activators such as p53 and VP16. In human, TAF9 and AK6 (GeneID: 102157402) are two distinct genes that share 5' exons. A similar but distinct gene (TAF9L) has been found on the X chromosome and a pseudogene has been identified on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Canonical amino-acid sequenceUniProt

264 residues, UniProt reviewed canonical sequence.

>Q16594|TAF9
     1  MESGKTASPK SMPKDAQMMA QILKDMGITE YEPRVINQML EFAFRYVTTI LDDAKIYSSH
    61  AKKATVDADD VRLAIQCRAD QSFTSPPPRD FLLDIARQRN QTPLPLIKPY SGPRLPPDRY
   121  CLTAPNYRLK SLQKKASTSA GRITVPRLSV GSVTSRPSTP TLGTPTPQTM SVSTKVGTPM
   181  SLTGQRFTVQ MPTSQSPAVK ASIPATSAVQ NVLINPSLIG SKNILITTNM MSSQNTANES
   241  SNALKRKRED DDDDDDDDDD YDNL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TAF9 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.63

Highest tissue expression

128 nTPM

Expression across tissuesHPA

ReferencesPubMed · IEDB

Publications for TAF9 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.83

gnomAD pLI

0

gnomAD missense Z

1.02

DepMap mean gene effect

-0.13

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• box C/D snoRNP assembly
• chromatin remodeling
• DNA damage response
• mRNA transcription by RNA polymerase II
• negative regulation of apoptotic process
• negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
• negative regulation of proteasomal ubiquitin-dependent protein catabolic process
• positive regulation of DNA-templated transcription
• positive regulation of response to cytokine stimulus
• positive regulation of transcription by RNA polymerase II
• positive regulation of transcription initiation by RNA polymerase II
• protein stabilization
• regulation of DNA repair
• regulation of DNA-templated transcription
• regulation of RNA splicing
• regulation of transcription by RNA polymerase II
• response to interleukin-1
• RNA polymerase II preinitiation complex assembly

Molecular functions

• ATPase binding
• C2H2 zinc finger domain binding
• DNA binding
• DNA-binding transcription factor binding
• p53 binding
• protein heterodimerization activity
• RNA polymerase II general transcription initiation factor activity
• transcription cis-regulatory region binding
• transcription coactivator activity

Cellular components

• MLL1 complex
• nucleoplasm
• nucleus
• pre-snoRNP complex
• SAGA complex
• transcription factor TFIID complex
• transcription factor TFTC complex

Protein domainsUniProt · Pfam · InterPro

• Transcription initiation factor TAFII31
• Histone-fold
• Transcription initiation factor TFIID subunit 9/TAF9
• Transcription initiation factor IID, 31kD subunit

KeywordsUniProt

• Acetylation
• DNA-binding
• Nucleus
• Phosphoprotein
• Transcription
• Transcription regulation

InteractionsUniProt · HPA

Protein binding partners of TAF9 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• TAF5
• ZNHIT6
• DRAP1
• KAT2B
• KPNA1
• KPNA6
• RPS14
• SGF29
• SUPT20H
• TADA1
• TADA2B
• TAF6L