Seroatlas · Human Serome Atlas

TAF5

Transcription initiation factor TFIID subunit 5

Also known as: TAF2D, TAF5_HUMAN, TAFII100

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q15542
Gene: TAF5
Ensembl: ENSG00000148835
Chromosome: 10
Canonical length: 800 aa
Protein class: Plasma proteins, Predicted intracellular proteins
Subcellular location: Nucleoplasm
Quaternary structure: Homodimer

OverviewNCBI Gene

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes an integral subunit of TFIID associated with all transcriptionally competent forms of that complex. This subunit interacts strongly with two TFIID subunits that show similarity to histones H3 and H4, and it may participate in forming a nucleosome-like core in the TFIID complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Canonical amino-acid sequenceUniProt

800 residues, UniProt reviewed canonical sequence.

>Q15542|TAF5
     1  MAALAEEQTE VAVKLEPEGP PTLLPPQAGD GAGEGSGGTT NNGPNGGGGN VAASSSTGGD
    61  GGTPKPTVAV SAAAPAGAAP VPAAAPDAGA PHDRQTLLAV LQFLRQSKLR EAEEALRREA
   121  GLLEEAVAGS GAPGEVDSAG AEVTSALLSR VTASAPGPAA PDPPGTGASG ATVVSGSASG
   181  PAAPGKVGSV AVEDQPDVSA VLSAYNQQGD PTMYEEYYSG LKHFIECSLD CHRAELSQLF
   241  YPLFVHMYLE LVYNQHENEA KSFFEKFHGD QECYYQDDLR VLSSLTKKEH MKGNETMLDF
   301  RTSKFVLRIS RDSYQLLKRH LQEKQNNQIW NIVQEHLYID IFDGMPRSKQ QIDAMVGSLA
   361  GEAKREANKS KVFFGLLKEP EIEVPLDDED EEGENEEGKP KKKKPKKDSI GSKSKKQDPN
   421  APPQNRIPLP ELKDSDKLDK IMNMKETTKR VRLGPDCLPS ICFYTFLNAY QGLTAVDVTD
   481  DSSLIAGGFA DSTVRVWSVT PKKLRSVKQA SDLSLIDKES DDVLERIMDE KTASELKILY
   541  GHSGPVYGAS FSPDRNYLLS SSEDGTVRLW SLQTFTCLVG YKGHNYPVWD TQFSPYGYYF
   601  VSGGHDRVAR LWATDHYQPL RIFAGHLADV NCTRFHPNSN YVATGSADRT VRLWDVLNGN
   661  CVRIFTGHKG PIHSLTFSPN GRFLATGATD GRVLLWDIGH GLMVGELKGH TDTVCSLRFS
   721  RDGEILASGS MDNTVRLWDA IKAFEDLETD DFTTATGHIN LPENSQELLL GTYMTKSTPV
   781  VHLHFTRRNL VLAAGAYSPQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TAF5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.36
Highest tissue expression: 8.9 nTPM

Expression across tissuesHPA

Tissue

testis: 8.9 nTPM
thymus: 4.4 nTPM
tonsil: 2.7 nTPM
lymph node: 2.4 nTPM
tongue: 2.3 nTPM
placenta: 2.2 nTPM

Single-cell type

early primary spermatocytes: 72 nCPM
differentiating spermatogonia: 45 nCPM
undifferentiated spermatogonia: 43 nCPM
late primary spermatocytes: 25 nCPM
erythrocyte progenitors: 21 nCPM
late spermatids: 20 nCPM

Immune cell

T-reg: 0.6 nTPM
eosinophil: 0.3 nTPM
memory CD8 T-cell: 0.3 nTPM
naive B-cell: 0.3 nTPM
naive CD4 T-cell: 0.3 nTPM
classical monocyte: 0.2 nTPM

Brain region

cerebellum: 1.3 nTPM
cerebral cortex: 0.9 nTPM
hypothalamus: 0.7 nTPM
medulla oblongata: 0.7 nTPM
pons: 0.7 nTPM
amygdala: 0.6 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.21
gnomAD pLI: 1
gnomAD missense Z: 2.65
DepMap mean gene effect: -0.72
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of TAF5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TAF5 as an antibody target. Whether an autoantibody or antibody against TAF5 could matter depends on whether native TAF5 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TAF5 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TAF5 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...