DRAP1

Protein identityUniProt · HPA

UniProt accession

Q14919

Gene

DRAP1

Ensembl

ENSG00000175550

Chromosome

11

Canonical length

205 aa

Protein class

Predicted intracellular proteins

Subcellular location

Cytosol

OverviewNCBI Gene

Transcriptional repression is a general mechanism for regulating transcriptional initiation in organisms ranging from yeast to humans. Accurate initiation of transcription from eukaryotic protein-encoding genes requires the assembly of a large multiprotein complex consisting of RNA polymerase II and general transcription factors such as TFIIA, TFIIB, and TFIID. DR1 is a repressor that interacts with the TATA-binding protein (TBP) of TFIID and prevents the formation of an active transcription complex by precluding the entry of TFIIA and/or TFIIB into the preinitiation complex. The protein encoded by this gene is a corepressor of transcription that interacts with DR1 to enhance DR1-mediated repression. The interaction between this corepressor and DR1 is required for corepressor function and appears to stabilize the TBP-DR1-DNA complex. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

205 residues, UniProt reviewed canonical sequence.

>Q14919|DRAP1
     1  MPSKKKKYNA RFPPARIKKI MQTDEEIGKV AAAVPVIISR ALELFLESLL KKACQVTQSR
    61  NAKTMTTSHL KQCIELEQQF DFLKDLVASV PDMQGDGEDN HMDGDKGARR GRKPGSGGRK
   121  NGGMGTKSKD KKLSGTDSEQ EDESEDTDTD GEEETSQPPP QASHPSAHFQ SPPTPFLPFA
   181  STLPLPPAPP GPSAPDEEDE EDYDS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DRAP1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.61

Highest tissue expression

202 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.8

gnomAD pLI

0.13

gnomAD missense Z

0.87

DepMap mean gene effect

-0.36

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• negative regulation of transcription by RNA polymerase II
• transcription by RNA polymerase II

Molecular functions

• core promoter sequence-specific DNA binding
• identical protein binding
• protein heterodimerization activity
• RNA polymerase II general transcription initiation factor activity
• RNA polymerase II general transcription initiation factor binding
• TBP-class protein binding

Cellular components

• negative cofactor 2 complex
• nucleus
• RNA polymerase II transcription regulator complex

Protein domainsUniProt · Pfam · InterPro

• Transcription factor CBF/NF-Y/archaeal histone domain
• Histone-fold
• Transcription/DNA replication regulator
• Histone-like transcription factor (CBF/NF-Y) and archaeal histone

KeywordsUniProt

• DNA-binding
• Nucleus
• Phosphoprotein
• Repressor
• Transcription
• Transcription regulation

InteractionsUniProt · HPA

Protein binding partners of DRAP1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• TBP
• DR1
• NFYB
• POLE3
• TAF9