RPA4

Protein identityUniProt · HPA

UniProt accession

Q13156

Gene

RPA4

Ensembl

ENSG00000204086

Chromosome

X

Canonical length

261 aa

Protein class

Predicted intracellular proteins

Subcellular location

Nucleoplasm

OverviewNCBI Gene

This gene encodes a single-stranded DNA-binding protein that is the 30-kDa subunit of the replication protein A complex. Replication protein A is an essential factor for DNA double-strand break repair and cell cycle checkpoint activation. The encoded protein localizes to DNA repair foci and may be involved in the cellular DNA damage response. This protein may also play a role in inhibiting viral replication.[provided by RefSeq, Apr 2010]

Canonical amino-acid sequenceUniProt

261 residues, UniProt reviewed canonical sequence.

>Q13156|RPA4
     1  MSKSGFGSYG SISAADGASG GSDQLCERDA TPAIKTQRPK VRIQDVVPCN VNQLLSSTVF
    61  DPVFKVRGII VSQVSIVGVI RGAEKASNHI CYKIDDMTAK PIEARQWFGR EKVKQVTPLS
   121  VGVYVKVFGI LKCPTGTKSL EVLKIHVLED MNEFTVHILE TVNAHMMLDK ARRDTTVESV
   181  PVSPSEVNDA GDNDESHRNF IQDEVLRLIH ECPHQEGKSI HELRAQLCDL SVKAIKEAID
   241  YLTVEGHIYP TVDREHFKSA D

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RPA4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.4

Highest tissue expression

1.6 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD missense Z

0.25

DepMap mean gene effect

0.02

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• DNA damage checkpoint signaling
• DNA repair
• DNA replication
• DNA replication initiation
• double-strand break repair via homologous recombination
• nucleotide-excision repair

Molecular functions

• single-stranded DNA binding
• telomeric DNA binding

Cellular components

• chromosome, telomeric region
• DNA replication factor A complex
• nucleoplasm
• nucleus
• site of double-strand break

Protein domainsUniProt · Pfam · InterPro

• Nucleic acid-binding, OB-fold
• Replication factor A protein 2
• Replication protein A, C-terminal
• Winged helix-like DNA-binding domain superfamily
• Winged helix DNA-binding domain superfamily
• Replication factor A protein-like
• Replication protein A C terminal

KeywordsUniProt

• DNA damage
• DNA repair
• DNA-binding
• Nucleus

InteractionsUniProt · HPA

Protein binding partners of RPA4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• RPA1
• RPA3
• SUPT16H