Seroatlas · Human Serome Atlas

PYCARD

Apoptosis-associated speck-like protein containing a CARD

Also known as: ASC, ASC_HUMAN, CARD5, TMS-1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9ULZ3
Gene
PYCARD
Ensembl
ENSG00000103490
Chromosome
16
Canonical length
195 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm,Nucleoli,Cytosol
Quaternary structure
Homooligomer

OverviewNCBI Gene

This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

195 residues, UniProt reviewed canonical sequence.

>Q9ULZ3|PYCARD
     1  MGRARDAILD ALENLTAEEL KKFKLKLLSV PLREGYGRIP RGALLSMDAL DLTDKLVSFY
    61  LETYGAELTA NVLRDMGLQE MAGQLQAATH QGSGAAPAGI QAPPQSAAKP GLHFIDQHRA
   121  ALIARVTNVE WLLDALYGKV LTDEQYQAVR AEPTNPSKMR KLFSFTPAWN WTCKDLLLQA
   181  LRESQSYLVE DLERS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PYCARD can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Other membrane
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.36
Highest tissue expression
111 nTPM

Expression across tissuesHPA

Tissue

  • skin: 111 nTPM
  • spleen: 105 nTPM
  • esophagus: 69 nTPM
  • duodenum: 60 nTPM
  • small intestine: 59 nTPM
  • colon: 58 nTPM

Single-cell type

  • hofbauer cells: 540 nCPM
  • kupffer cells: 449 nCPM
  • enterocytes: 431 nCPM
  • monocytes: 363 nCPM
  • esophageal suprabasal cells: 329 nCPM
  • neutrophils: 328 nCPM

Immune cell

  • neutrophil: 1,647 nTPM
  • non-classical monocyte: 1,159 nTPM
  • intermediate monocyte: 1,140 nTPM
  • classical monocyte: 1,102 nTPM
  • eosinophil: 951 nTPM
  • myeloid DC: 831 nTPM

Brain region

  • white matter: 22 nTPM
  • medulla oblongata: 15 nTPM
  • spinal cord: 14 nTPM
  • thalamus: 13 nTPM
  • pons: 12 nTPM
  • hypothalamus: 9.3 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.9
gnomAD pLI
0
gnomAD missense Z
0.4
DepMap mean gene effect
0.02
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of PYCARD in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PYCARD as an antibody target. Whether an autoantibody or antibody against PYCARD could matter depends on whether native PYCARD is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PYCARD is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PYCARD as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/PYCARD. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

© 2026 · Seroatlas, Discover Oregon LLC · Terms of use

Loading the interactive Seroatlas protein explorer...